PXD037041

PXD037041 is an original dataset announced via ProteomeXchange.

Title	MASP micro-specimen samples at different spatial locations
Description	Quantitative protein mapping on whole-tissue levels provides important insights into the spatially-organized regulatory processes/networks related to diseases and therapy, but remains a tremendous challenge. We describe a micro-scaffold assisted spatial proteomics(MASP) method, based on precise tissue spatial-compartmentalization using a 3D-printed micro-scaffold, capable of mapping thousands of proteins across a whole-tissue slice with excellent quantitative quality. The mapping accuracy was validated and applied in mapping >5,000 cerebral proteins in mouse brain. Under stringent cutoffs, 5019 unique proteins were mapped(N=208 micro-specimens) and 4577 proteins were mapped in all regions.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:37:39.370.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shihan Huo
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-27 11:36:00	ID requested
1	2023-03-10 23:01:10	announced
⏵ 2	2023-11-14 08:37:40	announced	2023-11-14: Updated project metadata.

Ma M, Huo S, Zhang M, Qian S, Zhu X, Pu J, Rasam S, Xue C, Shen S, An B, Wang J, Qu J, In-depth mapping of protein localizations in whole tissue by micro-scaffold assisted spatial proteomics (MASP). Nat Commun, 13(1):7736(2022) [pubmed]

submitter keyword: MASP,LC-MSMS

Jun Qu
contact affiliation	Department of Pharmaceutical Sciences, University at Buffalo, Buffalo, New York, United State
contact email	junqu@buffalo.edu
lab head
Shihan Huo
contact affiliation	University at Buffalo
contact email	shihanhu@buffalo.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD037041

PRIDE project URI

• PRIDE
  1. PXD037041
     1. Label: PRIDE project
     2. Name: MASP micro-specimen samples at different spatial locations