PXD037039

PXD037039 is an original dataset announced via ProteomeXchange.

Title	A platform for the recombinant production of Group A Streptococcus glycoconjugate vaccines
Description	Strep A are human-exclusive bacterial pathogens killing annually more than 500,000 patients, and no current licensed vaccine exists. Strep A bacteria are highly diverse, but all produce an essential, abundant, and conserved main surface carbohydrate, the Group A Carbohydrate (GAC). GAC contains a rhamnose polysaccharide (RhaPS) backbone, which is a validated universal vaccine candidate. RhaPS vaccine candidate production is currently limited to chemical routes. We engineered the GAC synthesis route to enable the industry standard recombinant production route to couple RhaPS to selected carrier proteins. The integrity of the correct chemical structure of the glycoconjugate vaccines was confirmed by NMR spectroscopy and mass spectrometry. Purified IdeS-RhaPS and NanA-RhaPS produce carbohydrate specific antibodies in mice and rabbits confirming the recombinantly produced RhaPS glycoconjugates as valuable vaccine candidates. We provide a promising route to the production of tailor-made low-cost glycoconjugate vaccines against Strep A and other bacterial pathogens.
HostingRepository	PRIDE
AnnounceDate	2025-04-22
AnnouncementXML	Submission_2025-04-22_05:32:03.124.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kathirvel Alagesan
SpeciesList	scientific name: Streptococcus pyogenes serotype M1; NCBI TaxID: 301447;
ModificationList	complex glycosylation
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-27 11:31:41	ID requested
⏵ 1	2025-04-22 05:32:03	announced

10.1038/S41541-025-01068-2;

submitter keyword: glycoconjugate,Streptococcus, Glycoproteomics

Kathirvel Alagesan
contact affiliation	Max Planck Unit for the Science of Pathogens, charitéplatz 1, D-10117 Berlin, Germany
contact email	alagesan@mpusp.mpg.de
lab head
Kathirvel Alagesan
contact affiliation	Max Planck Unit for the Science of Pathogens
contact email	alagesan@mpusp.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/04/PXD037039

PRIDE project URI

• PRIDE
  1. PXD037039
     1. Label: PRIDE project
     2. Name: A platform for the recombinant production of Group A Streptococcus glycoconjugate vaccines