PXD037034

PXD037034 is an original dataset announced via ProteomeXchange.

Title	Identification and functional characterization of proteins interacting with cohesin complex in yeast Saccharomyces cerevisiae.
Description	To ensure equal separation of DNA, sister chromatids are held together from S phase to metaphase–anaphase transition by a multiprotein complex called cohesin. This makes it possible to establish chromosome biorientation, counteracts the pulling force of mitotic spindle microtubules, preventing premature sister chromatid separation, and ensures precise segregation of sister DNAs into daughter cells In order to better understand how the sister chromatid cohesion process is regulated we looked for new cohesin interacors. We constructed a yeast strain endogenously expressing TAP-tagged Scc1 (Scc1-TAP). Next, we performed a single-step TAP purification using an untagged strain (mock sample) or a strain expressing Scc1-TAP, followed by identification of co-purifying proteins by MS.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:06:34.201.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD037034
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Agata Malinowska
SpeciesList	scientific name: Olea europaea (Common olive); NCBI TaxID: 4146; scientific name: Pan troglodytes (Chimpanzee); NCBI TaxID: 9598; scientific name: Brassica napus (Rape); NCBI TaxID: 3708; scientific name: Halobacterium salinarum; NCBI TaxID: 2242; scientific name: Candida albicans (Yeast); NCBI TaxID: 5476; scientific name: Equus caballus (Horse); NCBI TaxID: 9796; scientific name: Mesocricetus auratus (Golden hamster); NCBI TaxID: 10036; scientific name: Ictalurus punctatus (Channel catfish); NCBI TaxID: 7998; scientific name: Rattus rattus (Black rat); NCBI TaxID: 10117; scientific name: Taeniopygia guttata (Zebra finch) (Poephila guttata); NCBI TaxID: 59729; scientific name: Bombyx mori (Silk moth); NCBI TaxID: 7091; scientific name: Enterococcus faecalis (Streptococcus faecalis); NCBI TaxID: 1351; scientific name: Caenorhabditis elegans; NCBI TaxID: 6239; scientific name: Solanum tuberosum (Potato); NCBI TaxID: 4113; scientific name: Hepatitis C virus subtype 1a; NCBI TaxID: 31646; scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: 7955; scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227; scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Helicobacter pylori J99 (Campylobacter pylori J99); NCBI TaxID: 85963; scientific name: Merluccius bilinearis (Silver hake); NCBI TaxID: 79698; scientific name: Brassica oleracea var. botrytis (Cauliflower); NCBI TaxID: 3715; scientific name: Pongo pygmaeus (Bornean orangutan); NCBI TaxID: 9600; scientific name: Cicer arietinum (Chickpea) (Garbanzo); NCBI TaxID: 3827; scientific name: Cercopithecus aethiops (Green monkey) (Grivet); NCBI TaxID: 9534; scientific name: Pisum sativum (Garden pea); NCBI TaxID: 3888; scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825; scientific name: Heliocidaris erythrogramma (Sea urchin); NCBI TaxID: 7634; scientific name: Staphylococcus aureus; NCBI TaxID: 1280; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; scientific name: Nicotiana tabacum (Common tobacco); NCBI TaxID: 4097; scientific name: Ricinus communis; NCBI TaxID: 3988; scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Macaca mulatta (Rhesus macaque); NCBI TaxID: 9544; scientific name: Dictyostelium discoideum (Slime mold); NCBI TaxID: 44689; scientific name: Pinus radiata (Monterey pine); NCBI TaxID: 3347; scientific name: Gallus gallus (Chicken); NCBI TaxID: 9031; scientific name: Oryza sativa (Rice); NCBI TaxID: 4530; scientific name: Struthio camelus (Ostrich); NCBI TaxID: 8801; scientific name: Gadus morhua (Atlantic cod); NCBI TaxID: 8049; scientific name: Hordeum vulgare (Barley); NCBI TaxID: 4513;
ModificationList	methylthiolated residue; monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-27 11:27:34	ID requested
1	2023-10-24 10:21:49	announced
⏵ 2	2023-11-14 09:06:43	announced	2023-11-14: Updated project metadata.

Litwin I, Nowicka M, Markowska K, Maciaszczyk-Dziubi, ń, ska E, Tomaszewska P, Wysocki R, Kramarz K, ISW1a modulates cohesin distribution in centromeric and pericentromeric regions. Nucleic Acids Res, 51(17):9101-9121(2023) [pubmed]

10.6019/PXD037034;

submitter keyword: Scc1,cohesin complex, cohesion

Ireneusz Litwin
contact affiliation	University of Wrocław, Faculty of Biological Sciences
contact email	ireneusz.litwin@uwr.edu.pl
lab head
Agata Malinowska
contact affiliation	IBB PAS
contact email	esme@ibb.waw.pl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD037034

PRIDE project URI

• PRIDE
  1. PXD037034
     1. Label: PRIDE project
     2. Name: Identification and functional characterization of proteins interacting with cohesin complex in yeast Saccharomyces cerevisiae.