PXD037031 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Urinary endogenous peptides as biomarkers for prostate cancer |
Description | Prostate cancer (PCa) is one of the most prevalent cancer types in men worldwide. However, the main diagnostic tests available for PCa have limitations and need biopsy for histopathological confirmation of the disease. The prostate-specific antigen (PSA) is the main biomarker used for PCa early detection, but an elevated serum concentration is not cancer-specific. Therefore, there is a need for discovery of new non-invasive biomarkers that can accurately diagnose PCa. Here, we used trichloroacetic acid-induced protein precipitation and liquid chromatography-mass spectrometry to profile endogenous peptides in urine samples from patients with PCa (n = 33), benign prostatic hyperplasia (n = 25), and healthy individuals (n = 28). Receiver operating characteristic (ROC) curves were performed to evaluate the diagnostic performance of urinary peptides. In addition, proteasix tool was used for in silico prediction of protease cleavage sites. We found five urinary peptides derived from uromodulin significantly altered between the study groups, all of which were less abundant in the PCa group. In addition, urinary peptides outperformed PSA in discriminating between malignant and benign prostate conditions (AUC = 0.847), showing high sensitivity (81.82%) and specificity (88%). Overall, our study allowed the identification of urinary peptides with potential for use as non-invasive biomarkers in PCa diagnosis. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-08 |
AnnouncementXML | Submission_2024-10-08_11:36:04.558.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Cristine Dutra |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue |
Instrument | Q-Tof Ultima |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-09-27 11:26:45 | ID requested | |
⏵ 1 | 2024-10-08 11:36:04 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: uromodulin, urine, endogenous peptides, mass spectrometry, biomarkers,Prostate cancer |
Contact List
Karina Mariante Monteiro |
contact affiliation | Laboratory of Structural and Functional Genomics, Biotechnology Center, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil |
contact email | karina.monteiro@ufrgs.br |
lab head | |
Cristine Dutra |
contact affiliation | Universidade Federal do Rio Grande do Sul |
contact email | cristinedutra@hotmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD037031
- Label: PRIDE project
- Name: Urinary endogenous peptides as biomarkers for prostate cancer