PXD037027

PXD037027 is an original dataset announced via ProteomeXchange.

Title	Lysine lactylation regulates biofilm formation of streptococcus mutans
Description	Lactate produced in glycolysis has recently been discovered to modify lysine residues on eukaryotic proteins, a post-translational modification (PTM) called lysine lactylation (Kla). This modification not only participates in regulating gene expression through lactylation of histones, but also impacts the function of non-histone proteins. However, lactylation in prokaryotes has not been reported. Here, we report the identification of 1869 lysine lactylation sites in 465 proteins in the cariogenic organism Streptococcus mutans using a newly developed 4-dimensional label-free quantitative proteomic approach, representing the first Kla dataset in prokaryotes. Combining quantitative analysis, we report that the modification levels of 282 sites from 124 proteins increased by over 1.5-fold, and 80 sites from 52 proteins decreased by over 0.67-fold in the biofilm growth state. These results provide a systematic perspective of the distribution and function of Kla and improve our understanding of the role of lactate in the metabolic regulation of prokaryotes.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:11:26.853.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xian Peng
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	acetylated residue
Instrument	maXis

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-27 11:20:43	ID requested
1	2023-08-24 00:55:18	announced
⏵ 2	2023-11-14 07:11:27	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: Lactylation

Streptococcus mutans

Biofilm

Xian Peng
contact affiliation	State Key Laboratory of Oral Diseases, Sichuan University
contact email	pengx@scu.edu.cn
lab head
Xian Peng
contact affiliation	State Key Laboratory of Oral Diseases, Sichuan University
contact email	pengx@scu.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD037027
     1. Label: PRIDE project
     2. Name: Lysine lactylation regulates biofilm formation of streptococcus mutans