PXD037021

PXD037021 is an original dataset announced via ProteomeXchange.

Title	Tear proteome revealed association of S100A family proteins and mesothelin with thrombosis in elderly patients with retinal vein occlusion
Description	Tear samples collected from patients with central retinal vein occlusion (n=58) and group of healthy volunteers (n=30) were analyzed using a proteomic label-free absolute quantitative ap-proach. We found a large proportion (458 proteins with a frequency > 0.6) of tear proteome shared between healthy donors and subjects with the central retinal vein occlusion (CRVO). Comparative proteomic analysis revealed 29 proteins (p<0.05) significantly differed in their quantitative property between CRVO patients and the control group. Among them S100A6 (log (2)FC = 1.11, p<0.001), S100A8 (log(2)FC = 2.45, p<0.001), and S100A9 (log2(FC) = 2.08, p<0.001) are the most abundantly represented upregulated proteins, and β2-microglobulin was the most downregulated protein (log2(FC) = -2.13, p<0.001). Mesothelin (MSLN) was found as a protein with a significant increase (log2(FC) = 0.82, p<0.001) in the CRVO group. The selected up- and downregulated pro-teins were assembled to customize map of the CRVO-related critical protein interactions with quantitative properties. The customized map (FDR<0.01) emerged inflammation, impairment of retinal hemostasis, and immune response as the main set of processes associated with CRVO is-chemic condition. The semantic analysis encouraged the prevalence of core biological processes encompassing dysregulation of mitochondrial organization and regulation of improperly or utilization of topologically incorrect folded proteins as a consequence of oxidative stress and in-flammation escalating the ischemic condition caused by the local retinal hemostasis dysregulation. The most significantly different proteins (S100A6, S100A8, S100A9, MSLN, and β2-microglobulin) were applied for the ROC plotting and their AUC varied from 0.772 to 0.952 suggesting association with the CRVO.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:41:27.701.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD037021
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Arthur Kopylov
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-pyridylethyl-L-cysteine
Instrument	Xevo G2-XS Tof

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-27 08:33:46	ID requested
1	2024-10-08 11:36:04	announced
⏵ 2	2024-10-22 05:41:28	announced	2024-10-22: Updated project metadata.

10.6019/PXD037021;

submitter keyword: retinal vein occlusion

retinal hemostasis

S100A protein

mesothelin

inflammation

Anna L. Kaysheva
contact affiliation	Institute of Biomedical Chemistry, 119121 Moscow, 10 Pogodinskaya str., bld. 8, Russian Federation; tel.: +7 495 246 16 41; web: www.ibmc.msk.ru
contact email	kaysheva1@gmail.com
lab head
Arthur Kopylov
contact affiliation	Institute of Biomedical Chemistry
contact email	a.t.kopylov@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD037021
     1. Label: PRIDE project
     2. Name: Tear proteome revealed association of S100A family proteins and mesothelin with thrombosis in elderly patients with retinal vein occlusion