PXD037014

PXD037014 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Quantitative proteomic analysis of extracellular membrane vesicles from Acinetobacter baumannii (AbOMVs) isolated under normoxic and hypoxic conditions.
Description	Acinetobacter baumannii were cultured in Brain Heart Infusion medium (BD Bioscience). For normoxic condition, the cultures were shaken at 125 rpm for 24 h, whereas for hypoxic condition, the cultures were placed under static condition for 48 h. Outer membrane vesicles from A. baumannii (AbOMVs) in the culture supernatant from normoxic and hypoxic conditions (referred to nAbOMV and hAbOMVs, respectively) were harvested by ultracentrifugation at 100,000 × g, 4℃ for 90 min using a Himac CP80WX Preparative Ultracentrifuge (HITACHI, Tokyo, Japan). After removal of the supernatant, the pellet was washed twice and suspended in ice-cold sterile phosphate-buffered saline (PBS). AbOMVs were then isolated from other damaged membranes, aggregated proteins and non-membranous proteins by step-gradient ultracentrifugation using 15 to 45% OptiPrep™ Density Medium (Sigma Aldrich, St. Louis, MO). After centrifugation at 100,000 × g, 4℃ for 16 h, the substances in all six fractions (F1-F6) were collected, diluted in PBS, and harvested by ultracentrifugation at 100,000 × g, 4℃ for 3 h. The pellet from each fraction was washed twice and resuspended in a suitable volume of ice-cold sterile PBS.
HostingRepository	jPOST
AnnounceDate	2023-03-16
AnnouncementXML	Submission_2023-03-15_17:12:23.591.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yota Tatara
SpeciesList	scientific name: Acinetobacter baumannii; NCBI TaxID: 470;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine
Instrument	TripleTOF 6600

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-26 03:37:04	ID requested
⏵ 1	2023-03-15 17:12:24	announced

Publication List

Suzuki S, Subsomwong P, Narita K, Kawai N, Ishiai T, Teng W, Sukchawalit R, Nakane A, Tasaka S, Asano K, Differential proteomic analysis and pathogenic effects of outer membrane vesicles derived from Acinetobacter baumannii under normoxia and hypoxia. PLoS One, 18(3):e0283109(2023) [pubmed]

Suzuki S, Subsomwong P, Narita K, Kawai N, Ishiai T, Teng W, Sukchawalit R, Nakane A, Tasaka S, Asano K, Differential proteomic analysis and pathogenic effects of outer membrane vesicles derived from Acinetobacter baumannii under normoxia and hypoxia. PLoS One, 18(3):e0283109(2023) [pubmed]

Keyword List

submitter keyword: Acinetobacter baumannii, extracellular membrane vesicles, hypoxic

submitter keyword: Acinetobacter baumannii, extracellular membrane vesicles, hypoxic

Contact List

Krisana Asano
lab head
Yota Tatara
contact affiliation	Hirosaki University
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001865/