PXD036997 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | MiPRISMA: Interactome profiling of small peptides and young microproteins translated from human short open reading frames |
Description | All species continuously evolve small open reading frames (sORFs) that can be templated for protein synthesis and may provide raw material for evolutionary adaptation. We analyzed the evolutionary origins of 7,264 recently cataloged human sORFs and found that most were evolutionary young and emerged de novo. We additionally discovered 221 yet uncataloged peptides smaller than 16 amino acids, for which we found evidence of translation in human and rodent tissues. To investigate the potential bioactivity of microproteins and peptides translated from young and very small ORFs we established MiPRISMA: a mass spectrometry-based interactome screen with sequence motif resolution. We assessed 266 candidates and implicated several in essential cellular processes including splicing, translational regulation, and endocytosis, a subset of which we validate in cellular assays. MiPRISMA provides a scalable platform to characterize small and evolutionarily young proteins, shedding light on a largely unexplored territory of the putative human proteome. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:58:05.918.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Marieluise Kirchner |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF-X |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-09-25 15:10:52 | ID requested | |
1 | 2023-02-17 09:37:44 | announced | |
⏵ 2 | 2023-11-14 08:58:06 | announced | 2023-11-14: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: microproteins, small peptides, human,PRM |
Contact List
Philipp Mertins |
contact affiliation | Berlin Institute of Health at Charite, Universitaetsmedizin Berlin, Core Facility Proteomics, Max Delbrueck Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany |
contact email | philipp.mertins@mdc-berlin.de |
lab head | |
Marieluise Kirchner |
contact affiliation | Proteomics Platform, BIH@Charite |
contact email | marieluise.kirchner@mdc-berlin.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/02/PXD036997 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD036997
- Label: PRIDE project
- Name: MiPRISMA: Interactome profiling of small peptides and young microproteins translated from human short open reading frames