<<< Full experiment listing

PXD036997

PXD036997 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMiPRISMA: Interactome profiling of small peptides and young microproteins translated from human short open reading frames
DescriptionAll species continuously evolve small open reading frames (sORFs) that can be templated for protein synthesis and may provide raw material for evolutionary adaptation. We analyzed the evolutionary origins of 7,264 recently cataloged human sORFs and found that most were evolutionary young and emerged de novo. We additionally discovered 221 yet uncataloged peptides smaller than 16 amino acids, for which we found evidence of translation in human and rodent tissues. To investigate the potential bioactivity of microproteins and peptides translated from young and very small ORFs we established MiPRISMA: a mass spectrometry-based interactome screen with sequence motif resolution. We assessed 266 candidates and implicated several in essential cellular processes including splicing, translational regulation, and endocytosis, a subset of which we validate in cellular assays. MiPRISMA provides a scalable platform to characterize small and evolutionarily young proteins, shedding light on a largely unexplored territory of the putative human proteome.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:58:05.918.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMarieluise Kirchner
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF-X
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-09-25 15:10:52ID requested
12023-02-17 09:37:44announced
22023-11-14 08:58:06announced2023-11-14: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: microproteins, small peptides, human,PRM
Contact List
Philipp Mertins
contact affiliationBerlin Institute of Health at Charite, Universitaetsmedizin Berlin, Core Facility Proteomics, Max Delbrueck Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany
contact emailphilipp.mertins@mdc-berlin.de
lab head
Marieluise Kirchner
contact affiliationProteomics Platform, BIH@Charite
contact emailmarieluise.kirchner@mdc-berlin.de
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/02/PXD036997
PRIDE project URI
Repository Record List
[ + ]