PXD036991

PXD036991 is an original dataset announced via ProteomeXchange.

Title	Label-free quantitative proteomics of gastric high-grade intraepithelial neoplasia
Description	Purpose: Early detection and diagnosis are the keys to improving the survival rate and reducing the fatality rate linked to gastric cancer. The precancerous lesion of gastric cancer is referred to as Gastric high-grade intraepithelial neoplasia (HGIN). Both sensitivity and specificity of current biomarkers that aid in the diagnosis of gastric HGIN are still relatively low. The proteomic data of gastric HGIN are still scarce. The study aimed to explore candidate protein biomarkers for gastric HGIN screening with proteomics and bioinformatics technology. Methods: A total of 10 serum samples were collected and categorized into two groups, that is, the gastric HGIN and the healthy control groups. Label-free quantification (LFQ) in conjunction with liquid chromatography with tandem mass spectrometry (LC-MS/MS) was employed to identify the probable biomarkers for gastric HGIN. Further, differentially expressed proteins (DEPs) were quantified by the proteomic analysis. Results: In total, 1192 distinct serum proteins were discovered between the gastric HGIN group and the healthy control group. DEPs were recruited in the conduction of further analyses, utilizing a threshold of 1.5-fold difference in expression level (P ＜ 0.05) in comparison with the control group. There were 18 upregulated and 12 downregulated proteins in the gastric HGIN group in contrast with the control group. Bioinformatics analyses were performed according to Gene Ontology (GO), cluster, and KEGG pathway enrichment analyses. Conclusions: Our data provide more biological information for the formation of gastric HGIN and provide clues for further research on the pathogenesis of early gastric cancer.
HostingRepository	PRIDE
AnnounceDate	2025-06-02
AnnouncementXML	Submission_2025-06-01_16:08:09.977.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nan Lin
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-25 14:57:57	ID requested
⏵ 1	2025-06-01 16:08:10	announced

Lin N, Lin L, Huang X, Huang C, Gong J, Label-free quantitative proteomics of gastric high-grade intraepithelial neoplasia. Exp Ther Med, 30(1):133(2025) [pubmed]

10.3892/etm.2025.12883;

submitter keyword: gastric cancer

gastric high-grade intraepithelial neoplasia

proteomics

label-free

Nan Lin
contact affiliation	The Affiliated Hospital of Putian University
contact email	642803499@qq.com
lab head
Nan Lin
contact affiliation	the Affiliated Hospital of Putian University
contact email	642803499@qq.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/06/PXD036991

PRIDE project URI

• PRIDE
  1. PXD036991
     1. Label: PRIDE project
     2. Name: Label-free quantitative proteomics of gastric high-grade intraepithelial neoplasia