PXD036981

PXD036981 is an original dataset announced via ProteomeXchange.

Title	The influence of symbiosis on the proteome of the symbiotic dinoflagellate Breviolum
Description	The physiology and cellular mechanisms of the dinoflagellate symbionts of cnidarians, the Symbiodiniaceae, change as a response to symbiosis, nutrient availability, and their surrounding microhabitat, but the underlying processes are poorly understood. Here, we employed liquid chromatography–mass spectrometry-based proteomics to elucidate the changes associated with the symbiotic and nutritional states of Breviolum minutum, a native symbiont of the sea anemone Exaiptasia diaphana. Both symbiosis and nutritional state had significant impacts on the B. minutum proteome. B. minutum in hospite showed increased abundance of proteins that are involved in phosphoinositol manipulation (e.g., glycerophosphoinositol permease 1 and phosphatidylinositol phosphatase), potentially as a means of interpartner signalling to prevent host phagosomal maturation. Proteins involved in carbon concentration and fixation (e.g. carbonic anhydrase, V-type ATPase) and nitrogen assimilation (e.g. glutamine synthase) were upregulated in ex hospite B. minutum, possibly due to nitrogen limitation by host in hospite and a lack of host carbon concentration mechanisms when ex hospite, respectively. Photosystem proteins increased in abundance at high nutrient levels, as were proteins involved in antioxidant mechanisms (e.g. superoxide dismutase, glutathione s-transferase). Proteins involved in iron metabolism was also affected by nutrient state, indicating increased iron demand and uptake in low nutrient treatments. These results provide better insight on the cellular mechanisms of symbiosis and provides potential target pathways for investigating a functional cnidarian-dinoflagellate symbiosis.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:59:30.100.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD036981
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Clinton Oakley
SpeciesList	scientific name: Breviolum minutum; NCBI TaxID: 2499525;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-25 14:35:42	ID requested
1	2023-01-30 20:28:18	announced
⏵ 2	2023-11-14 08:59:30	announced	2023-11-14: Updated project metadata.

10.6019/PXD036981;

submitter keyword: dinoflagellate, Aiptasia, coral, algae,symbiosis, Breviolum

Simon Davy
contact affiliation	School of Biological Sciences, Victoria University of Wellington
contact email	simon.davy@vuw.ac.nz
lab head
Clinton Oakley
contact affiliation	Victoria University of Wellington
contact email	clint.oakley@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD036981
     1. Label: PRIDE project
     2. Name: The influence of symbiosis on the proteome of the symbiotic dinoflagellate Breviolum