PXD036974

PXD036974 is an original dataset announced via ProteomeXchange.

Title	Mammalian oocytes store mRNAs in a mitochondria-associated membraneless compartment
Description	Fully-grown oocytes are transcriptionally silent and must stably maintain mRNAs needed for oocyte meiotic maturation and early embryonic development. However, where and how mammalian oocytes store maternal mRNAs is unclear. Here, we report that mammalian oocytes accumulate mRNAs in a mitochondria-associated ribonucleoprotein domain (MARDO). MARDO assembly around mitochondria was promoted by the RNA-binding protein ZAR1, and directed by an increase in mitochondrial membrane potential during oocyte growth. MARDO foci coalesced into hydrogel-like matrices that clustered mitochondria. Maternal mRNAs stored in the MARDO were translationally repressed. Loss of ZAR1 disrupted the MARDO, dispersed mitochondria, and caused a premature loss of MARDO-localized mRNAs. Thus, a mitochondria-associated membraneless compartment controls mitochondrial distribution and regulates maternal mRNA storage, translation and decay to ensure fertility in mammals.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:43:34.107.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Luisa Welp
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-25 14:31:16	ID requested
1	2022-10-25 09:33:46	announced
⏵ 2	2023-11-14 07:43:35	announced	2023-11-14: Updated project metadata.

Cheng S, Altmeppen G, So C, Welp LM, Penir S, Ruhwedel T, Menelaou K, Harasimov K, St, ü, tzer A, Blayney M, Elder K, M, ö, bius W, Urlaub H, Schuh M, Mammalian oocytes store mRNAs in a mitochondria-associated membraneless compartment. Science, 378(6617):eabq4835(2022) [pubmed]

submitter keyword: Mouse, Oocytes, LC-ESI-MS/MS, DDA, mRNA storage

Melina Schuh
contact affiliation	Department of Meiosis, Max Planck Institute for Multidisciplinary Sciences; Göttingen, Germany
contact email	melina.schuh@mpinat.mpg.de
lab head
Luisa Welp
contact affiliation	Max-Planck-Institute for Biophysical Chemistry
contact email	lwelp@gwdg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD036974

PRIDE project URI

• PRIDE
  1. PXD036974
     1. Label: PRIDE project
     2. Name: Mammalian oocytes store mRNAs in a mitochondria-associated membraneless compartment