PXD036973

PXD036973 is an original dataset announced via ProteomeXchange.

Title	Sialylation of vesicular integrin β1 of bladder cancer cells promotes its binding to fibronectin, and endocytic entry of small extracellular vesicles into recipient epithelial cells
Description	Small extracellular vesicles (sEV) are closely associated with the development and metastasis of many types of mammalian cancer. Glycoconjugates are highly expressed on sEV and play important roles. However, functions in sEV of sialic acids, the terminal component of glycoconjugates, are poorly understood. We observed greatly increased sialic acid levels in sEV from bladder cancer cells. These sEV promoted the oncogenic transformation of recipient normal bladder epithelial cells. When sialic acids on sEV were eliminated, sEV uptake by recipient cells was significantly reduced. Sialylation of vesicular integrin β1 was found to play a key role in sEV uptake. Desialylation downstream of the hybrid domain of vesicular integrin β1 inhibited its binding to matrix fibronectin, reduced sEV entry into recipient cells, and suppressed metastasis of those cells. Our findings indicate important functional roles of sialic acids in sEV uptake and reprogramming plasticity of surrounding normal epithelial cells.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:40:32.134.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD036973
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Meixuan Lin
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-25 14:27:32	ID requested
1	2024-05-21 12:04:49	announced
⏵ 2	2024-10-22 06:40:34	announced	2024-10-22: Updated project metadata.

Lin M, Xu X, Zhou X, Feng H, Wang R, Yang Y, Li J, Fan N, Jiang Y, Li X, Guan F, Tan Z, 1 determined endocytic entry of small extracellular vesicles into recipient cells. Cell Mol Biol Lett, 29(1):46(2024) [pubmed]

10.6019/PXD036973;

10.1186/s11658-024-00562-0;

submitter keyword: Sialic acid, bladder cancer, small extracellular vesicles, integrin β1

Feng Guan
contact affiliation	College of Life Sciences, Northwest University, 229 Taibai North Road, Xi’an, Shaanxi 710069, China
contact email	guanfeng@nwu.edu.cn
lab head
Meixuan Lin
contact affiliation	Northwest University
contact email	202010248@stumail.nwu.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD036973
     1. Label: PRIDE project
     2. Name: Sialylation of vesicular integrin β1 of bladder cancer cells promotes its binding to fibronectin, and endocytic entry of small extracellular vesicles into recipient epithelial cells