PXD036957
PXD036957 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Unbiased proteomics profiling of pulmonary immune cells originating from irradiated lungs, non-irradiated lungs, or lungs from control mice |
Description | One murine lung was irradiated using 6Gyx5 X-ray exposure. Following irradiation, CD45+ (immune) were sorted from the ipsilateral (irradiated) and contralateral (non-irradiated) lung. Cells from non-irradiated controls were also collected. Proteins from the immune cells were extracted using the MPLEx method. Proteins were reduced with DTT, alkylated with Iodoacetamide, and digested using trypsin. 5 ul of 0.1 ug/ul of peptides were analyzed by LC-MS/MS on a Thermo Q Exactive Orbitrap instrument. |
HostingRepository | MassIVE |
AnnounceDate | 2023-02-20 |
AnnouncementXML | Submission_2023-02-20_14:27:57.802.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Matthew Monroe |
SpeciesList | scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090; |
ModificationList | Carbamidomethyl; Oxidation |
Instrument | Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2022-09-23 11:21:00 | ID requested | |
⏵ 1 | 2023-02-20 14:27:58 | announced |
Publication List
no publication |
Keyword List
submitter keyword: lung, irradiation, immune cells |
Contact List
Geremy Clair | |
---|---|
contact affiliation | Pacific Northwest National Laboratory |
contact email | geremy.clair@pnnl.gov |
lab head | |
Matthew Monroe | |
contact affiliation | Pacific Northwest National Laboratory |
contact email | matthew.monroe@pnnl.gov |
dataset submitter |
Full Dataset Link List
MassIVE dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000090379/ |