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PXD036957

PXD036957 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleUnbiased proteomics profiling of pulmonary immune cells originating from irradiated lungs, non-irradiated lungs, or lungs from control mice
DescriptionOne murine lung was irradiated using 6Gyx5 X-ray exposure. Following irradiation, CD45+ (immune) were sorted from the ipsilateral (irradiated) and contralateral (non-irradiated) lung. Cells from non-irradiated controls were also collected. Proteins from the immune cells were extracted using the MPLEx method. Proteins were reduced with DTT, alkylated with Iodoacetamide, and digested using trypsin. 5 ul of 0.1 ug/ul of peptides were analyzed by LC-MS/MS on a Thermo Q Exactive Orbitrap instrument.
HostingRepositoryMassIVE
AnnounceDate2023-02-20
AnnouncementXMLSubmission_2023-02-20_14:27:57.802.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterMatthew Monroe
SpeciesList scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationListCarbamidomethyl; Oxidation
InstrumentQ Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-09-23 11:21:00ID requested
12023-02-20 14:27:58announced
Publication List
no publication
Keyword List
submitter keyword: lung, irradiation, immune cells
Contact List
Geremy Clair
contact affiliationPacific Northwest National Laboratory
contact emailgeremy.clair@pnnl.gov
lab head
Matthew Monroe
contact affiliationPacific Northwest National Laboratory
contact emailmatthew.monroe@pnnl.gov
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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