PXD036955

PXD036955 is an original dataset announced via ProteomeXchange.

Title	Genetically Encoded Activity-Based Ubiquitin and Ubiquitin-like Protein Probes
Description	Post-translational modification of proteins by Ubiquitin (Ub) and Ubiquitin-like proteins (Ubls) can be reversed by deconjugating enzymes, which have been implicated in different pathways and associated with various human diseases. To understand the activity and dynamics of deconjugating enzymes, multiple synthetic and semi-synthetic Ub/Ubl probes have been developed, and some of them have been applied to screen inhibitors of deconjugating enzymes. Since these Ub/Ubl probes are generally not cell-permeable, different strategies have been developed to deliver Ub/Ubl probes to live cells. However, till now no Ub/Ubl probes can be expressed in live cells to directly report on the activities of deconjugating enzymes in the most relevant cellular environment. Here, we genetically encoded cross-linkable Ub/Ubl probes in live E. coli and HEK293T cells. These probes can cross-link with deconjugating enzymes in vitro and in vivo, and the extent of cross-linking is dependent on the activities of deconjugating enzymes.
HostingRepository	iProX
AnnounceDate	2022-09-23
AnnouncementXML	Submission_2023-01-03_01:48:04.719.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xin Shu
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-23 00:04:09	ID requested
⏵ 1	2023-01-03 01:48:05	announced

Dataset with its publication pending

submitter keyword: Genetically Encoded, Activity-Based Ubiquitin Probe

Bing Yang
contact affiliation	Zhejiang University
contact email	bingyang@zju.edu.cn
lab head
Xin Shu
contact affiliation	Zhejiang University
contact email	sx725@zju.edu.cn
dataset submitter

iProX dataset URI