PXD036951

PXD036951 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomics informed tailored low-dose drug combination strategies in Pancreatic ductal adenocarcinoma cell lines
Description	Pancreatic ductal adenocarcinoma is a devastating disease with poor prognosis, commonly characterized by aberrant signaling. Phosphoproteomics provides a functional scaffold to unravel these complex signaling networks and to identify new targets. By a two-step sequential phospho-peptide enrichment, we generated the phosphoproteome for 9 PDAC cell lines (2 derived from PDX samples). By using integrative inferred kinase activity (INKA) scoring, we identified hyperactive phosphokinases that were subsequently matched to kinase inhibitors. In the absence of an oncogenic driver, low-dose kinase inhibitor combinations against multiple (parallel) activated kinases, provided higher efficacy as compared to single-drug treatment. Tailored low-dose combination strategies exhibited promising efficacy in vitro and in vivo, which may ultimately improve treatment outcomes in PDAC patients.
HostingRepository	PRIDE
AnnounceDate	2023-06-01
AnnouncementXML	Submission_2023-06-01_08:33:37.320.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	SanderPiersma
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-22 05:51:59	ID requested
1	2023-05-09 05:18:05	announced
⏵ 2	2023-06-01 08:33:37	announced	2023-06-01: Updated project metadata.

Dataset with its publication pending

submitter keyword: PDAC, IMAC, single shot, kinase, phosphoproteomics, pTyr IP, INKA

ConnieJimenez
contact affiliation	AmsterdamUMC, Vrije Universiteit Amsterdam, Medical Oncology, Cancer Center Amsterdam, OncoProteomics Laboratory, Amsterdam, The Netherlands
contact email	c.jimenez@amsterdamumc.nl
lab head
SanderPiersma
contact affiliation	Amsterdam UMC
contact email	s.piersma@amsterdamumc.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD036951

PRIDE project URI

• PRIDE
  1. PXD036951
     1. Label: PRIDE project
     2. Name: Phosphoproteomics informed tailored low-dose drug combination strategies in Pancreatic ductal adenocarcinoma cell lines