PXD036939 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | MHC-IP MS of EpiScan A02 SARS-CoV-2 library |
Description | Identification of CD8+ T-cell epitopes is critical for the development of immunotherapeutics. Existing methods for MHC-I ligand discovery are time-intensive, specialized and unable to interrogate specific proteins on a large scale. Here we present EpiScan, which uses surface MHC-I levels as a readout for whether a genetically encoded peptide is an MHC-I ligand. Oligonucleotide synthesis permits facile screening for MHC-I ligands amongst predetermined starting pools comprising >100,000 peptides. We exploit this programmability of EpiScan to uncover an unappreciated role for cysteine that increases the number of predicted ligands by 12-21%, reveal affinity hierarchies by analysis of biased-anchor peptide libraries, and screen viral proteomes for MHC-I ligands. Using these data, we generate and iteratively refine peptide binding prediction predictions to create EpiScan Predictor, or ESP. ESP performed comparably to other state-of-the-art MHC-I peptide binding prediction algorithms while not suffering from underrepresentation of cysteine-containing peptides. Thus, targeted immunopeptidomics using EpiScan will accelerate CD8+ T-cell epitope discovery towards the goal of patient-specific immunotherapeutics. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:40:13.713.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Peter Bruno |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Severe acute respiratory syndrome coronavirus 2; NCBI TaxID: 2697049; |
ModificationList | acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-09-22 00:12:33 | ID requested | |
1 | 2022-10-13 06:05:34 | announced | |
⏵ 2 | 2024-10-22 05:40:14 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
ProteomeXchange project tag: Sars-cov-2, Covid-19 |
submitter keyword: EpiScan MHC-IP SARS-CoV-2 HLA-A*02:01 |
Contact List
Stephen Elledge |
contact affiliation | Division of Genetics, Department of Medicine, Brigham and Women's Hospital |
contact email | selledge@genetics.med.harvard.edu |
lab head | |
Peter Bruno |
contact affiliation | Brigham and Women's Hospital |
contact email | pbruno@bwh.harvard.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD036939
- Label: PRIDE project
- Name: MHC-IP MS of EpiScan A02 SARS-CoV-2 library