PXD036904

PXD036904 is an original dataset announced via ProteomeXchange.

Title	Formation of ER-lumenal intermediates during export of Plasmodium proteins containing transmembrane-like hydrophobic sequences
Description	REX3RQLSE:Pf332:C-S11:DSLE is exported and is diffusely localised in the red blood cell cytoplasm (Fig 3A). Mutation of the PEXEL sequence (REX3AQLSE:Pf332:C-S11:DSLE), results in the protein being retained in the parasite (Fig 3C), and addition of a C-terminal SDEL sequence (REX3RQLSE:Pf332:C-S11:SDEL) also leads to retention of the protein within the parasite (Fig 3B). Given that REX3RQLSE:Pf332:C-S11:SDEL is retained within the parasite, this indicates that the C-terminus of the protein is in the ER lumen. To determine the location of the N-terminal end of this ER-retained protein, it was purified for tryptic digestion and analysis by mass spectrometry. The most N-terminal peptides retrieved corresponded to N-acetylated and non-acetylated SEPVVEEQDLK and SEPVVEEQDLKK. These peptides correspond to the expected N-terminal sequence following PEXEL cleavage by Plasmepsin V, indicating that the N-terminus of this protein is also in the ER-lumen. These data indicate that both N- and C-terminal ends of REX3RQLSE:Pf332:C-S11:SDEL are located within the ER lumen and consequently that in the context of this protein, the putative transmembrane segment of Pf332 has a propensity to translocate into the ER lumen rather than integrate into the ER membrane.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:44:48.680.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Georgina Charlton
SpeciesList	scientific name: Plasmodium falciparum; NCBI TaxID: 5833;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse; Synapt G2-S MS

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-21 14:48:05	ID requested
1	2023-03-28 06:53:03	announced
2	2023-04-11 05:01:10	announced	2023-04-11: Updated project metadata.
⏵ 3	2023-11-14 08:44:53	announced	2023-11-14: Updated project metadata.

Levray YS, Bana B, Tarr SJ, McLaughlin EJ, Rossi-Smith P, Waltho A, Charlton GH, Chiozzi RZ, Straton CR, Thalassinos K, Osborne AR, Formation of ER-lumenal intermediates during export of Plasmodium proteins containing transmembrane-like hydrophobic sequences. PLoS Pathog, 19(3):e1011281(2023) [pubmed]

submitter keyword: Plasmodium, Proteomics, LC-MS/MS

Konstantinos Thalassinos
contact affiliation	Institute of Structural and Molecular Biology, University College London, Gower Street, London WC1E 6BT, United Kingdom
contact email	k.thalassinos@ucl.ac.uk
lab head
Georgina Charlton
contact affiliation	University College London
contact email	georgina.charlton@ucl.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD036904
     1. Label: PRIDE project
     2. Name: Formation of ER-lumenal intermediates during export of Plasmodium proteins containing transmembrane-like hydrophobic sequences