PXD036890

PXD036890 is an original dataset announced via ProteomeXchange.

Title	RNP remodeling during RNA virus replication through phosphoregulation of SR proteins revealed by metabolic labeling
Description	Mechanisms used by positive-stranded RNA viruses to control the translation-replication switch remain largely unexplored. Using metabolic labeling coupled with quantitative proteomics analyses, we unraveled the protein composition of temporal ribonucleoprotein complexes (RNPs) isolated during early and late enterovirus A71 (EV-A71) infection. Comparing the RNP components at the eclipse and maturation phase, representing early and late infection, revealed RNP remodeling over time by exchanging nuclear with cytoplasmic proteins. Among the nuclear proteins, EV-A71 infection induced the phosphorylation and cytoplasmic re-localization of nuclear SR proteins. During early infection, phosphorylated SR proteins cofractionated with the translation machinery rather than the replication organelles. During late infection, this co-localization, as well as the EV-A71-induced phosphorylated SR proteins, was no longer detected. Inhibition of SR protein phosphorylation by the kinase inhibitors SRPKIN-1 and TG003 significantly reduced the replication of several enteroviruses. Our results demonstrate the importance of phosphoregulation of SR proteins during enterovirus replication and reveal a potential target for broad-spectrum antivirals.
HostingRepository	PRIDE
AnnounceDate	2025-06-04
AnnouncementXML	Submission_2025-06-03_19:10:59.118.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chih-Ching Wu
SpeciesList	scientific name: Enterovirus A71; NCBI TaxID: NCBITaxon:39054; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LCQ Classic

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-21 01:08:02	ID requested
⏵ 1	2025-06-03 19:10:59	announced

Dataset with its publication pending

ProteomeXchange project tag: Human Proteome Project

submitter keyword: 4sU labeling, EV-A71,Ribonucleotide protein, SR protein

Chih-Ching Wu
contact affiliation	Chang Gung University Department of Medical Biotechnology and Laboratory Science
contact email	luckywu@mail.cgu.edu.tw
lab head
Chih-Ching Wu
contact affiliation	Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University
contact email	luckywu@mail.cgu.edu.tw
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD036890
     1. Label: PRIDE project
     2. Name: RNP remodeling during RNA virus replication through phosphoregulation of SR proteins revealed by metabolic labeling