PXD036876

PXD036876 is an original dataset announced via ProteomeXchange.

Title	Bacillus subtilis remains translationally active after CRISPRi-mediated replication initiation arrest
Description	Initiation of bacterial DNA replication takes place at the origin of replication (oriC), a region characterized by the presence of multiple DnaA boxes that serve as the binding sites for the master initiator protein DnaA. The absence or failure of DNA replication can result in bacterial cell growth arrest or death. Here, we aimed to uncover the physiological and molecular consequences of stopping replication in the model bacterium Bacillus subtilis. For this purpose, DNA replication was blocked using a CRISPRi approach specifically targeting DnaA boxes 6 and 7, which are essential for replication initiation. We characterized the phenotype of these cells and analyzed the overall changes in the proteome using quantitative mass spectrometry. Cells with arrested replication were elongating and not dividing but showed no evidence of DNA damage response (DDR). Moreover, these cells did not cease translation over time. This study sets the ground for future research on non-replicating but translationally active B. subtilis, which might be valuable for biotechnological applications.
HostingRepository	PRIDE
AnnounceDate	2024-03-26
AnnouncementXML	Submission_2024-03-26_01:52:02.517.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kathirvel Alagesan
SpeciesList	scientific name: Bacillus subtilis PY79; NCBI TaxID: 1415167;
ModificationList	carbamoylated residue; monohydroxylated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-21 00:53:54	ID requested
⏵ 1	2024-03-26 01:52:02	announced

10.1128/MSYSTEMS00221-24;

submitter keyword: Bacillus subtilis, DnaA boxes, oriC, replication, CRISPRi

Kürşad Turgay
contact affiliation	Max Planck Unit for the Science of Pathogens, D-10117 Berlin, Germany Institute of Microbiology, Leibniz Universität Hannover, Hannover, Germany
contact email	turgay@mpusp.mpg.de
lab head
Kathirvel Alagesan
contact affiliation	Max Planck Unit for the Science of Pathogens
contact email	alagesan@mpusp.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD036876
     1. Label: PRIDE project
     2. Name: Bacillus subtilis remains translationally active after CRISPRi-mediated replication initiation arrest