PXD036875

PXD036875 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomic profiling of androgenic sexual endocrine perturbation in zebrafish embryos
Description	Endocrine disruption (ED) can trigger far-reaching effects on environmental populations, justifying a refusal of market approval for chemicals with ED properties. For the assessment of ED effects on development and reproduction, regulatory decisions mostly rely on apical endpoints from in vivo testing with adult animals. Here, we present a rapid and reproducible data dependent proteomics approach for identifying comprehensive molecular fingerprints interfering with the sexual endocrine system in zebrafish (Danio rerio) embryos as an alternative to animal testing. For this, we have analysed methyltestosterone as model substances for androgenic perturbation in a modified zebrafish embryo toxicity test (zFET). These signatures allow for a definition of solid biomarkers as tools in screening approaches and for integration in chronic toxicity studies for identifying suspect ED substances, in the fish early life-stage test (OECD TG 210).
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:29:44.048.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Steve Ayobahan
SpeciesList	scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: 7955;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-21 00:53:21	ID requested
1	2023-02-25 01:01:07	announced
⏵ 2	2023-11-14 08:29:46	announced	2023-11-14: Updated project metadata.

Ayobahan SU, Alvincz J, Reinwald H, Strompen J, Salinas G, Sch, ä, fers C, Eilebrecht E, Eilebrecht S, Comprehensive identification of gene expression fingerprints and biomarkers of sexual endocrine disruption in zebrafish embryo. Ecotoxicol Environ Saf, 250():114514(2023) [pubmed]

submitter keyword: Zebrafish embryos, Endocrine disruptors, LC-MS/MS

Dr. Sebastian Eilebrecht
contact affiliation	Fraunhofer Institute for Molecular Biology and Applied Ecology IME Auf dem Aberg 1 | 57392 Schmallenberg | Germany
contact email	sebastian.eilebrecht@ime.fraunhofer.de
lab head
Steve Ayobahan
contact affiliation	Fraunhofer IME
contact email	steve.ayobahan@ime.fraunhofer.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/02/PXD036875

PRIDE project URI

• PRIDE
  1. PXD036875
     1. Label: PRIDE project
     2. Name: Quantitative proteomic profiling of androgenic sexual endocrine perturbation in zebrafish embryos