PXD036866

PXD036866 is an original dataset announced via ProteomeXchange.

Title	Shotgun Proteomics Associates Mobile Phone Usage With Molecular-Level Responses Involved In Brain Functioning, Tissue Remodeling, Reproduction And Calcium Absorption Part 5
Description	Background: Mobile phones have become ubiquitous. There are concerns about possible health hazards posed by mobile phone-based radio frequency electromagnetic field (RF-EMF). Such exposure may be recognized as external stressor at molecular level which can be identified up by shot gun proteomics approach. Objective: To study molecular effects of mobile phone-based RF-EMF exposure using shot gun proteomics. Methods: 40 individuals (30 mobile phone users and 10 non-users) were recruited based on the inclusion and exclusion criteria. Mobile phone users had mobile phone usage of more than a year, an age group of 18-65 years with no major medical or surgical illness and normal results of hemogram, liver function tests (LFT), kidney function tests (KFT) and random/fasting sugars. Non-users had no mobile phone, Wi-Fi, and microwave usage. Both mobile phone users and non-users were non-alcoholic and non-smoker. RF-EMF exposure from mobile phone and miscellaneous sources was assessed using questionnaire. Calling-time duration per day was used as a measure of mobile phone exposure and the users were classified accordingly. Five biological replicates (1 set with both genders, 2 sets each of males and females) of proteomics experiments were designed. Human plasma samples were immunodepleted of high abundant proteins, concentrated, reduced, alkylated, digested, and labelled by i-TRAQ reagents for mass spectrometry analysis. Bioinformatics study was done on differentially expressed proteins for gene ontology (GO) classification, Protein-protein interactions (PPIs) and pathway enrichment. Results: The study group was quite young (median age 24 years). Pathway enrichment studies found Dopamine mediated receptor signaling, Adrenaline and Noradrenalin synthesis, Plasminogen activating cascade, Cholecystokinin receptor (CCKR) signaling, Gonadotropin-releasing hormone (GnRH) pathway and Vitamin-D signaling and its metabolism to be implicated. Conclusion: The involvement of the differentially expressed proteins in various signalling cascades have implications physiologically albeit large sample size studies incorporating non-users need to be conducted to draw out clear inferences.
HostingRepository	PRIDE
AnnounceDate	2026-06-07
AnnouncementXML	Submission_2026-06-07_04:00:22.469.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	NEELANCHAL VAID
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iTRAQ4plex-116 reporter+balance reagent acylated residue
Instrument	TripleTOF 5600; TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-20 14:54:12	ID requested
⏵ 1	2026-06-07 04:00:22	announced

Dataset with its publication pending

submitter keyword: Mobile phone, Vitamin-D signaling., Plasminogen activating cascade, Adrenaline and Noradrenalin synthesis, i-TRAQ, Shot gun proteomics, Cholecystokinin receptor signaling, Radio frequency electromagnetic fields, Dopamine mediated receptor signaling, Gonadotropin-releasing hormone pathway

DR. MANISH SONEJA
contact affiliation	DEPARTMENT OF MEDICINE, ALL INDIA INSTITUTE OF MEDICAL SCIENCES, ANSARI NAGAR, NEW DELHI-110029
contact email	manishsoneja@gmail.com
lab head
NEELANCHAL VAID
contact affiliation	ALL INDIA INSTITUTE OF MEDICAL SCIENCES
contact email	vaidneel@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD036866
     1. Label: PRIDE project
     2. Name: Shotgun Proteomics Associates Mobile Phone Usage With Molecular-Level Responses Involved In Brain Functioning, Tissue Remodeling, Reproduction And Calcium Absorption Part 5