PXD036857

PXD036857 is an original dataset announced via ProteomeXchange.

Title	CG14435 (detour), the Drosophila ZNRF1 and 2 homologue, regulates autophagosome biogenesis critical for healthy aging
Description	Autophagy is essential for animal development and homeostasis, and its deregulation is associated with numerous human pathologies. Here we identify a novel regulator of autophagy, the RING domain containing ubiquitin ligase CG14435 (detour). Depletion of CG14435 resulted in increased early-stage autophagic puncta, premature tissue contraction, and an accumulation of autophagy related proteins in young adults. Overexpression of CG14435 or the mammalian homologues, Zinc and RING finger (ZNRF) 1 and ZNRF2, increased late-stage autophagy puncta size. The ablation of ZNRF1 or ZNRF2 in mammalian cells increased basal autophagy. We identified CG14435 interacting proteins including HOPS complex subunits, deep orange (Vps18), Vps16A, and light (Vps41). Furthermore, in the presence of CG14435, dor ubiquitination was increased. The CG14435 mutant adults displayed premature aging, impaired motor function (neurodegeneration), and activation of innate immunity. Our findings reveal a novel role for CG14435 in autophagy, likely through regulation of HOPS complex, required for healthy aging.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:40:14.355.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jarrod Sandow
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	No PTMs are included in the dataset
Instrument	maXis

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-20 14:38:03	ID requested
1	2024-05-21 09:34:34	announced
⏵ 2	2024-10-22 06:40:20	announced	2024-10-22: Updated project metadata.

Nicolson S, Manning JA, Lim Y, Jiang X, Kolze E, Dayan S, Umargamwala R, Xu T, Sandow JJ, Webb AI, Kumar S, Denton D, The Drosophila ZNRF1/2 homologue, detour, interacts with HOPS complex and regulates autophagy. Commun Biol, 7(1):183(2024) [pubmed]

10.1038/s42003-024-05834-1;

submitter keyword: autophagy, Drosophila, aging

Donna Denton
contact affiliation	UniSA
contact email	donna.denton@unisa.edu.au
lab head
Jarrod Sandow
contact affiliation	WEHI
contact email	sandow@wehi.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD036857

PRIDE project URI

• PRIDE
  1. PXD036857
     1. Label: PRIDE project
     2. Name: CG14435 (detour), the Drosophila ZNRF1 and 2 homologue, regulates autophagosome biogenesis critical for healthy aging