PXD036841
PXD036841 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Polybrominated diphenyl ethers interact with the key protein involved in carbohydrate metabolism in rice |
Description | Rice exposed to organic pollutants such as polybrominated diphenyl ethers (PBDEs) usually experiences reduced biomass and increased soluble sugar content. This study showed that 2, 2', 4, 4'-tetrabromodiphenyl ether (BDE-47) led to increased glucose, fructose, and sucrose in rice leaves, accompanied by decreased photosynthetic rate and biomass. In order to identify the key enzyme that BDE-47 interacted with, a diazirine-alkynyl photoaffinity probe was designed, and photoaffinity labeling based chemoproteomics was conducted. Among all differentially expressed proteins, fructose-1, 6-bisphosphate aldolase (FBA) involved in carbohydrate metabolism was most likely the target protein of BDE-47. Spectral techniques and molecular docking analysis further revealed that the pollutant-protein interaction was driven by hydrophobic force. BDE-47 inhibited FBA catalytic efficiency by competing with its substrate, fructose-1, 6-diphosphate (F-1, 6-P), leading to soluble sugar accumulation, photosynthetic rate decline and biomass reduction. This study unraveled the influencing mechanism of PBDEs on rice by combining the novel photoaffinity labeling-based chemoproteomics with conventional proteomics. The improved knowledge on direct interaction between organic pollutants and proteins will help alleviate the harmful effects of soil pollution on plants. |
HostingRepository | iProX |
AnnounceDate | 2022-09-18 |
AnnouncementXML | Submission_2022-10-26_23:35:14.907.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Qian Liu |
SpeciesList | scientific name: Oryza sativa Indica Group x Oryza sativa Japonica Group; NCBI TaxID: 1050722; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2022-09-18 23:44:05 | ID requested | |
⏵ 1 | 2022-10-26 23:35:15 | announced |
Publication List
Liu Q, Liu N, Lu H, Yuan W, Zhu L, Polybrominated diphenyl ethers interact with the key protein involved in carbohydrate metabolism in rice. Environ Pollut, 316(Pt 1):120466(2023) [pubmed] |
Keyword List
submitter keyword: Soluble sugar, Biomass, Photoaffinity probe, Chemoproteomics, Fructose-1, 6-bisphosphate aldolase |
Contact List
Lizhong Zhu | |
---|---|
contact affiliation | Zhejiang University |
contact email | ZLZ@zju.edu.cn |
lab head | |
Qian Liu | |
contact affiliation | Zhejiang University |
contact email | 11914033@zju.edu.cn |
dataset submitter |
Full Dataset Link List
iProX dataset URI |