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PXD036837

PXD036837 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomics of db/db mice kidney
DescriptionKidney tissue samples were ground and pulverized in liquid nitrogen and transferred into low protein binding tubes and lysed with 300 µL lysis buffer supplemented with 1 mM PMSF. Then, samples were further lysed with sonication on ice. The parameters were set as 1s/1s intervals, 3 min, and 80 Watts of power. After sonication, samples were centrifuged at 12,000 g for 10 min at room temperature to remove insoluble particles that were repeated once to further exclude precipitation. Protein concentration was determined by Bradford assay and the samples were aliquoted and stored at -80 °C. Total proteins (15 µg) of each sample were acquired and separated by 12% SDS-PAGE gel. All samples were then trypsinized and labeled. The labeling peptide solutions were lyophilized and stored at -80 °C. Protein separation was performed on an 1100 HPLC System (Agilent) using an Agilent Zorbax Extend RP column (5 μm, 150 mm × 2.1 mm). Tryptic peptides were separated at a flow rate of 300 μL/min and monitored at 210 and 280 nm. Dried samples were harvested from 8 min to 50 min and elution buffer was collected every minute and numbered from 1-10 with the pipeline. The separated peptides were lyophilized for MS detection. The MS/MS data were analyzed for protein identification and quantification using Proteome Discoverer TM 2.2 (ThermoFisher Crop.). The local false discovery rate was estimated with the integrated PSPEP tool in the ProteinPilot Software to be 1.0% after searching against a decoy concatenated uniport Mus musculus protein database.
HostingRepositoryiProX
AnnounceDate2022-09-19
AnnouncementXMLSubmission_2022-12-07_23:17:25.047.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterShulan Su
SpeciesList scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-09-18 23:35:17ID requested
12022-09-18 23:35:30announced
22022-12-07 23:17:25announced2022-12-08: Update publication information.
Publication List
Xu Z, Xiang X, Su S, Zhu Y, Yan H, Guo S, Guo J, Shang EX, Qian D, Duan JA, . Front Pharmacol, 13():987668(2022) [pubmed]
Keyword List
submitter keyword: Proteomics
Contact List
Shulan Su
contact affiliationNanjing University of Traditional Chinese Medicine
contact emailsushulan1974@163.com
lab head
Shulan Su
contact affiliationNanjing University of Traditional Chinese Medicine
contact emailsushulan1974@163.com
dataset submitter
Full Dataset Link List
iProX dataset URI