PXD036818

PXD036818 is an original dataset announced via ProteomeXchange.

Title	FAM46C is an IFN stimulated gene that restricts lentiviral particle production by modulating autophagy
Description	FAM46C is a multiple myeloma tumour suppressor whose function is only starting to be elucidated. We recently showed that in multiple myeloma cells FAM46C triggers apoptosis by inhibiting autophagy and altering intracellular trafficking and protein secretion. To date, both a physiological characterization of FAM46C role and assessment of FAM46C-induced phenotypes outside of multiple myeloma are lacking. Preliminary reports suggested an involvement of FAM46C with regulation of viral replication but results were scanty and contradictory. Here, we show that FAM46C is an IFN-stimulated gene and that expression in HEK 293T cells of wt FAM46C but not of its most frequently found mutant variants, inhibits replication of both HIV-1 and HIV-1-derived lentiviruses. We demonstrate that this effect does not require transcriptional regulation and does not depend on inhibition of either global or viral-specific translation, but rather mostly relies on FAM46C-induced deregulation of autophagy, a pathway which we show to be required for efficient lentiviral production. These studies not only provide new insights on the physiological role of the FAM46C protein but could also help implementing more efficient antiviral strategies.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:49:04.568.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	alessandro cuomo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-17 15:04:57	ID requested
1	2023-06-19 07:44:18	announced
⏵ 2	2024-10-22 05:49:04	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: FAM46C inhibits lentiviral production

Nicola Manfrini
contact affiliation	INGM, Istituto Nazionale Genetica Molecolare "Romeo ed Enrica Invernizzi", Milan, Italy.
contact email	manfrini@ingm.org
lab head
alessandro cuomo
contact affiliation	IEO
contact email	alessandro.cuomo@ieo.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/06/PXD036818

PRIDE project URI

• PRIDE
  1. PXD036818
     1. Label: PRIDE project
     2. Name: FAM46C is an IFN stimulated gene that restricts lentiviral particle production by modulating autophagy