⮝ Full datasets listing

PXD036818

PXD036818 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleFAM46C is an IFN stimulated gene that restricts lentiviral particle production by modulating autophagy
DescriptionFAM46C is a multiple myeloma tumour suppressor whose function is only starting to be elucidated. We recently showed that in multiple myeloma cells FAM46C triggers apoptosis by inhibiting autophagy and altering intracellular trafficking and protein secretion. To date, both a physiological characterization of FAM46C role and assessment of FAM46C-induced phenotypes outside of multiple myeloma are lacking. Preliminary reports suggested an involvement of FAM46C with regulation of viral replication but results were scanty and contradictory. Here, we show that FAM46C is an IFN-stimulated gene and that expression in HEK 293T cells of wt FAM46C but not of its most frequently found mutant variants, inhibits replication of both HIV-1 and HIV-1-derived lentiviruses. We demonstrate that this effect does not require transcriptional regulation and does not depend on inhibition of either global or viral-specific translation, but rather mostly relies on FAM46C-induced deregulation of autophagy, a pathway which we show to be required for efficient lentiviral production. These studies not only provide new insights on the physiological role of the FAM46C protein but could also help implementing more efficient antiviral strategies.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:49:04.568.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitteralessandro cuomo
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-09-17 15:04:57ID requested
12023-06-19 07:44:18announced
22024-10-22 05:49:04announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: FAM46C inhibits lentiviral production
Contact List
Nicola Manfrini
contact affiliationINGM, Istituto Nazionale Genetica Molecolare "Romeo ed Enrica Invernizzi", Milan, Italy.
contact emailmanfrini@ingm.org
lab head
alessandro cuomo
contact affiliationIEO
contact emailalessandro.cuomo@ieo.it
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/06/PXD036818
PRIDE project URI
Repository Record List
[ + ]