PXD036818 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | FAM46C is an IFN stimulated gene that restricts lentiviral particle production by modulating autophagy |
Description | FAM46C is a multiple myeloma tumour suppressor whose function is only starting to be elucidated. We recently showed that in multiple myeloma cells FAM46C triggers apoptosis by inhibiting autophagy and altering intracellular trafficking and protein secretion. To date, both a physiological characterization of FAM46C role and assessment of FAM46C-induced phenotypes outside of multiple myeloma are lacking. Preliminary reports suggested an involvement of FAM46C with regulation of viral replication but results were scanty and contradictory. Here, we show that FAM46C is an IFN-stimulated gene and that expression in HEK 293T cells of wt FAM46C but not of its most frequently found mutant variants, inhibits replication of both HIV-1 and HIV-1-derived lentiviruses. We demonstrate that this effect does not require transcriptional regulation and does not depend on inhibition of either global or viral-specific translation, but rather mostly relies on FAM46C-induced deregulation of autophagy, a pathway which we show to be required for efficient lentiviral production. These studies not only provide new insights on the physiological role of the FAM46C protein but could also help implementing more efficient antiviral strategies. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:49:04.568.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | alessandro cuomo |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-09-17 15:04:57 | ID requested | |
1 | 2023-06-19 07:44:18 | announced | |
⏵ 2 | 2024-10-22 05:49:04 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: FAM46C inhibits lentiviral production |
Contact List
Nicola Manfrini |
contact affiliation | INGM, Istituto Nazionale Genetica Molecolare "Romeo ed Enrica Invernizzi", Milan, Italy. |
contact email | manfrini@ingm.org |
lab head | |
alessandro cuomo |
contact affiliation | IEO |
contact email | alessandro.cuomo@ieo.it |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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[ - ]
- PRIDE
- PXD036818
- Label: PRIDE project
- Name: FAM46C is an IFN stimulated gene that restricts lentiviral particle production by modulating autophagy