PXD036801 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Discovery of RUF6 ncRNA interacting proteins involved in P. falciparum immune evasion |
Description | RUF6 is a ncRNA gene family that is transcribed by RNA Polymerase III but actively regulates the Pol II-transcribed var virulence gene family. Understanding how RUF6 ncRNA connect to downstream effectors is lacking. We developed an RNA-directed proteomic discovery (ChIRP-MS) protocol to identify in vivo RUF6 ncRNA protein interactions. The RUF6 ncRNA interactome was purified with biotinylated antisense oligonucleotides. Quantitative label-free mass spectrometry identified several unique proteins linked to gene transcription including. Affinity purification of Pf-DDX5 identified proteins originally found by our RUF6-ChIRP protocol, validating the technique’s robustness for identifying ncRNA interactomes in P. falciparum. Our work identifies a RUF6 ncRNA protein complex that interacts with RNA Pol II to sustain var gene expression. |
HostingRepository | PRIDE |
AnnounceDate | 2022-11-15 |
AnnouncementXML | Submission_2022-11-15_05:11:04.020.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | VanessaMasson |
SpeciesList | scientific name: Plasmodium falciparum (isolate 3D7); NCBI TaxID: 36329; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-09-17 13:46:55 | ID requested | |
⏵ 1 | 2022-11-15 05:11:04 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: var genes,Malaria virulence, RUF6 ncRNA, Plasmodium falciparum, antigenic variation |
Contact List
DamarysLoew |
contact affiliation | Head of the Curie Institute Mass Spectrometry Platform |
contact email | damarys.loew@curie.fr |
lab head | |
VanessaMasson |
contact affiliation | Institut Curie - Centre de Recherche |
contact email | vanessa.masson@curie.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD036801
- Label: PRIDE project
- Name: Discovery of RUF6 ncRNA interacting proteins involved in P. falciparum immune evasion