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PXD036801

PXD036801 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDiscovery of RUF6 ncRNA interacting proteins involved in P. falciparum immune evasion
DescriptionRUF6 is a ncRNA gene family that is transcribed by RNA Polymerase III but actively regulates the Pol II-transcribed var virulence gene family. Understanding how RUF6 ncRNA connect to downstream effectors is lacking. We developed an RNA-directed proteomic discovery (ChIRP-MS) protocol to identify in vivo RUF6 ncRNA protein interactions. The RUF6 ncRNA interactome was purified with biotinylated antisense oligonucleotides. Quantitative label-free mass spectrometry identified several unique proteins linked to gene transcription including. Affinity purification of Pf-DDX5 identified proteins originally found by our RUF6-ChIRP protocol, validating the technique’s robustness for identifying ncRNA interactomes in P. falciparum. Our work identifies a RUF6 ncRNA protein complex that interacts with RNA Pol II to sustain var gene expression.
HostingRepositoryPRIDE
AnnounceDate2022-11-15
AnnouncementXMLSubmission_2022-11-15_05:11:04.020.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterVanessaMasson
SpeciesList scientific name: Plasmodium falciparum (isolate 3D7); NCBI TaxID: 36329;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-09-17 13:46:55ID requested
12022-11-15 05:11:04announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: var genes,Malaria virulence, RUF6 ncRNA, Plasmodium falciparum, antigenic variation
Contact List
DamarysLoew
contact affiliationHead of the Curie Institute Mass Spectrometry Platform
contact emaildamarys.loew@curie.fr
lab head
VanessaMasson
contact affiliationInstitut Curie - Centre de Recherche
contact emailvanessa.masson@curie.fr
dataset submitter
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Dataset FTP location
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