PXD036788

PXD036788 is an original dataset announced via ProteomeXchange.

Title	The small, high-light-induced protein SliP4 is involved in the proper organization of photosynthetic complexes in thylakoid membranes of Synechocystis sp. PCC 6803
Description	An increasing number of small proteins has been identified in the genomes of well-annotated model organisms including photosynthetic cyanobacteria such as Synechocystis sp. PCC 6803. A newly assigned protein comprising 37 amino acids was analyzed that is encoded upstream of the super-oxide dismutase SodB encoding gene. The initial hypothesis that this small protein might be functionally related to SodB could not be supported, instead we provide evidence that it fulfills important functions related to the organization of photosynthetic complexes. Therefore, we named it small light-induced protein of 4 kDa, SliP4. SliP4 is strongly induced under high-light-conditions and it is co-localized to photosystem I and II as well as NDH1 complexes on thylakoid membranes. Tagged SliP4 was used to purify NDH1 complexes, where it is especially associated with the subunits NdhF1 and NdhD1. Mutation of the protein-coding part in the sliP4 gene resulted in a high-light-sensitive phenotype, because the mutant was not further able to induce cyclic electron flow and state transitions under high-light conditions. Collectively our data indicate that SliP4 contributes to the association of NDH1 with the photosystem I and/or photosystem II complexes to facilitate different electron transfer modes under high light conditions.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:58:50.339.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sandra Maass
SpeciesList	scientific name: Synechocystis sp. PCC 6803; NCBI TaxID: 1148;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-17 02:43:06	ID requested
1	2023-06-07 03:53:37	announced
⏵ 2	2023-11-14 08:58:50	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: fluorescence, high light, photosynthesis, superoxide dismutase, NDH1, thylakoid,Acclimation

Dörte Becher
contact affiliation	University of Greifswald, Department of Microbial Proteomics, Institute of Microbiology, D-17489 Greifswald, Germany
contact email	dbecher@uni-greifswald.de
lab head
Sandra Maass
contact affiliation	University of Greifswald, Department for Microbial Proteomics
contact email	sandra.maass@uni-greifswald.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD036788
     1. Label: PRIDE project
     2. Name: The small, high-light-induced protein SliP4 is involved in the proper organization of photosynthetic complexes in thylakoid membranes of Synechocystis sp. PCC 6803