PXD036787

PXD036787 is an original dataset announced via ProteomeXchange.

Title	Serial passaging of Aspergillus fumigatus on Galleria extract agar produces strains with altered response of oxidative stress
Description	Aspergillus fumigatus is an environmental saprophyte and opportunistic fungal pathogen of the human lung. Serially passaging of A. fumigatus on an agar produced from Galleria mellonella larvae produced strains showing alterations in virulence, anti-fungal susceptibility and in protein abundances that may indicate a degree of adaptation or selection after 25 passages on the artificial agar. While passaged strains demonstrated reduced virulence in G. mellonella larvae, they demonstrated increased tolerance to haemocyte mediated killing, hydrogen peroxide, itraconazole and amphotericin B. Label-free proteomic analysis of control and passaged A. fumigatus strains revealed a total of 3329 proteins of which, 1902 remained following filtration and 32 proteins were statistically significant and differentially abundant. Proteins that play a role in the oxidative stress response were altered in abundance in the passaged strain and included (S)-S-oxide reductase (+2.63 fold), developmental regulator FlbA (+2.27 fold) and histone H2A.Z (-1.82 fold). The results presented here indicate that prolonged culturing of A. fumigatus on Galleria extract agar results in alterations in susceptibility to oxidative stress and in the abundance of proteins associated with the oxidative stress response. The phenomenon may be a result of selection for strains suitable for survival in the adverse conditions imposed by the innate immune response.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:45:21.111.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Aaron Curtis
SpeciesList	scientific name: Aspergillus fumigatus Af293; NCBI TaxID: 330879;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-17 02:42:27	ID requested
1	2023-05-10 08:50:23	announced
2	2023-11-14 08:45:51	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 05:45:21	announced	2024-10-22: Updated project metadata.

10.3390/cells12071065;

Curtis A, Walshe K, Kavanagh K, Extract Agar Results in Altered Virulence and Sensitivity to Antifungal Agents. Cells, 12(7):(2023) [pubmed]

submitter keyword: Aspergillus, Infection, Immunity, Virulence, Galleria, Mini-model, Passaging

Professor Kevin Kavanagh
contact affiliation	Medical Mycology Laboratory, Department of Biology, Maynooth University
contact email	kevin.kavanagh@mu.ie
lab head
Aaron Curtis
contact affiliation	Maynooth Univeristy
contact email	aaron.curtis.2018@mumail.ie
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD036787

PRIDE project URI

• PRIDE
  1. PXD036787
     1. Label: PRIDE project
     2. Name: Serial passaging of Aspergillus fumigatus on Galleria extract agar produces strains with altered response of oxidative stress