PXD036784

PXD036784 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Analysis of stalled ribosome-nascent chain complexes by quantitative proteomics
Description	The cellular environment is critical for efficient protein maturation, but how proteins fold during biogenesis remains poorly understood. To understand how the cellular environment modulates folding, we studied the cotranslational chaperone-assisted folding of Escherichia coli dihydrofolate reductase (DHFR). To sample folding intermediates along the pathway of vectorial synthesis, we prepared a series of stalled ribosome:nascent chain complexes (RNCs) representing snapshots of DHFR folding in vivo. Proteomic analysis of RNCs revealed their composition and allowed us to define chaperone interactions as a function of NC length. These data set the stage for further studies aimed at resolving the conformation of the nascent polypeptide on the ribosome.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_09:18:15.035.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Steven Howell
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-17 02:41:31	ID requested
⏵ 1	2025-05-06 09:18:15	announced

Publication List

10.1038/s41594-024-01355-x;
Wales TE, Pajak A, Roeselov, á A, Shivakumaraswamy S, Howell S, Kj, æ, r S, Hartl FU, Engen JR, Balchin D, Resolving chaperone-assisted protein folding on the ribosome at the peptide level. Nat Struct Mol Biol, 31(12):1888-1897(2024) [pubmed]

10.1038/s41594-024-01355-x;

Wales TE, Pajak A, Roeselov, á A, Shivakumaraswamy S, Howell S, Kj, æ, r S, Hartl FU, Engen JR, Balchin D, Resolving chaperone-assisted protein folding on the ribosome at the peptide level. Nat Struct Mol Biol, 31(12):1888-1897(2024) [pubmed]

Keyword List

submitter keyword: protein biosynthesis,ribosome-nascent chain complexes, interactome, protein folding, LC-MS/MS

submitter keyword: protein biosynthesis,ribosome-nascent chain complexes, interactome, protein folding, LC-MS/MS

Contact List

David Balchin
contact affiliation	Group leader, Protein Biogenesis Laboratory Francis Crick Institute 1 Midland Road London NW1 1AT
contact email	david.balchin@crick.ac.uk
lab head
Steven Howell
contact affiliation	Francis Crick Institute
contact email	steven.howell@crick.ac.uk
dataset submitter

Full Dataset Link List

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PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD036784

PRIDE project URI

Repository Record List

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