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PXD036784

PXD036784 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAnalysis of stalled ribosome-nascent chain complexes by quantitative proteomics
DescriptionThe cellular environment is critical for efficient protein maturation, but how proteins fold during biogenesis remains poorly understood. To understand how the cellular environment modulates folding, we studied the cotranslational chaperone-assisted folding of Escherichia coli dihydrofolate reductase (DHFR). To sample folding intermediates along the pathway of vectorial synthesis, we prepared a series of stalled ribosome:nascent chain complexes (RNCs) representing snapshots of DHFR folding in vivo. Proteomic analysis of RNCs revealed their composition and allowed us to define chaperone interactions as a function of NC length. These data set the stage for further studies aimed at resolving the conformation of the nascent polypeptide on the ribosome.
HostingRepositoryPRIDE
AnnounceDate2025-05-06
AnnouncementXMLSubmission_2025-05-06_09:18:15.035.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSteven Howell
SpeciesList scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationListiodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-09-17 02:41:31ID requested
12025-05-06 09:18:15announced
Publication List
10.1038/s41594-024-01355-x;
Wales TE, Pajak A, Roeselov, á A, Shivakumaraswamy S, Howell S, Kj, æ, r S, Hartl FU, Engen JR, Balchin D, Resolving chaperone-assisted protein folding on the ribosome at the peptide level. Nat Struct Mol Biol, 31(12):1888-1897(2024) [pubmed]
Keyword List
submitter keyword: protein biosynthesis,ribosome-nascent chain complexes, interactome, protein folding, LC-MS/MS
Contact List
David Balchin
contact affiliationGroup leader, Protein Biogenesis Laboratory Francis Crick Institute 1 Midland Road London NW1 1AT
contact emaildavid.balchin@crick.ac.uk
lab head
Steven Howell
contact affiliationFrancis Crick Institute
contact emailsteven.howell@crick.ac.uk
dataset submitter
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Dataset FTP location
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