PXD036784 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Analysis of stalled ribosome-nascent chain complexes by quantitative proteomics |
Description | The cellular environment is critical for efficient protein maturation, but how proteins fold during biogenesis remains poorly understood. To understand how the cellular environment modulates folding, we studied the cotranslational chaperone-assisted folding of Escherichia coli dihydrofolate reductase (DHFR). To sample folding intermediates along the pathway of vectorial synthesis, we prepared a series of stalled ribosome:nascent chain complexes (RNCs) representing snapshots of DHFR folding in vivo. Proteomic analysis of RNCs revealed their composition and allowed us to define chaperone interactions as a function of NC length. These data set the stage for further studies aimed at resolving the conformation of the nascent polypeptide on the ribosome. |
HostingRepository | PRIDE |
AnnounceDate | 2025-05-06 |
AnnouncementXML | Submission_2025-05-06_09:18:15.035.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Steven Howell |
SpeciesList | scientific name: Escherichia coli; NCBI TaxID: 562; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-09-17 02:41:31 | ID requested | |
⏵ 1 | 2025-05-06 09:18:15 | announced | |
Publication List
Keyword List
submitter keyword: protein biosynthesis,ribosome-nascent chain complexes, interactome, protein folding, LC-MS/MS |
Contact List
David Balchin |
contact affiliation | Group leader, Protein Biogenesis Laboratory Francis Crick Institute 1 Midland Road London NW1 1AT |
contact email | david.balchin@crick.ac.uk |
lab head | |
Steven Howell |
contact affiliation | Francis Crick Institute |
contact email | steven.howell@crick.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD036784
- Label: PRIDE project
- Name: Analysis of stalled ribosome-nascent chain complexes by quantitative proteomics