PXD036761
PXD036761 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | The Arabidopsis E3 ubiquitin ligase PUB4 regulates BIK1 and is targeted by a bacterial type-III effector |
| Description | Plant immunity is tightly controlled by a complex and dynamic regulatory network, which ensures optimal activation upon detection of potential pathogens. Accordingly, each component of this network is a potential target for manipulation by pathogens. Here, we report that RipAC, a type III-secreted effector from the bacterial pathogen Ralstonia solanacearum, targets the plant E3 ubiquitin ligase PUB4 to inhibit pattern-triggered immunity (PTI). PUB4 plays a positive role in PTI by regulating the homeostasis of the central immune kinase BIK1. Before PAMP perception, PUB4 promotes the degradation of non-activated BIK1, while, after PAMP perception, PUB4 contributes to the accumulation of activated BIK1. RipAC leads to BIK1 degradation, which correlates with its PTI-inhibitory activity. RipAC causes a reduction in pathogen-associated molecular pattern (PAMP)-induced PUB4 accumulation and phosphorylation. Our results shed light on the role played by PUB4 in immune regulation, and illustrate an indirect targeting of the immune signalling hub BIK1 by a bacterial effector. |
| HostingRepository | PanoramaPublic |
| AnnounceDate | 2022-10-12 |
| AnnouncementXML | Submission_2022-10-12_08:20:30.598.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | supported by repository but incomplete data and/or metadata |
| PrimarySubmitter | Frank Menke |
| SpeciesList | scientific name: Arabidopsis thaliana; NCBI TaxID: 3702; |
| ModificationList | Carbamidomethyl; Oxidation; Phospho |
| Instrument | Orbitrap Fusion |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2022-09-15 19:31:55 | ID requested | |
| ⏵ 1 | 2022-10-12 08:20:30 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Plant immunity, phosphorylation, effector |
Contact List
| Frank Menke | |
|---|---|
| contact affiliation | Sainsbury Laboratory |
| contact email | frank.menke@tsl.ac.uk |
| lab head | |
| Frank Menke | |
| contact affiliation | Sainsbury Laboratory |
| contact email | frank.menke@tsl.ac.uk |
| dataset submitter | |
Full Dataset Link List
| Panorama Public dataset URI |
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