PXD036755

PXD036755 is an original dataset announced via ProteomeXchange.

Title	Canopy light distribution effects on light use efficiency in wheat and its mechanism
Description	Optimizing canopy light distribution (CLD) has manifested improved light utility and yield without modifying other inputs. Nonetheless, molecular mechanisms working at cellular and organelle level remain to be elucidated. The current study aimed to assess the effect of CLD on photosynthetic performance and yield of wheat, and to investigate into the molecular mechanism underlying the photosynthetically active radiation (PAR) - use efficiency (PUE) at optimized CLD. Wheat was planted in two rows having different spacing [R1 (15 cm) and R2 (25 cm)] to simulate different CLD. Flag and penultimate leaves were subjected to chloroplast proteomics analysis. An increase in row spacing positively affects CLD. A decrease (16.64%) of PAR interception in the upper layer, an increase (19.76%) in the middle layer, improved PUE (12.08%) and increased yield (9.38%) were recorded. The abundance of proteins associated with photosynthetic electron transport, redox state, and carbon-nitrogen assimilation was differentially altered by CLD optimization. In the penultimate leaves, R2 reduced the abundance of PSII light-harvesting proteins, PSII-subunits, and increased the PSI light-harvesting proteins, NAD(P)H quinone oxidoreductase and enzymes involved in carbon assimilation compared to R1. Additionally, leaf stomatal conductance increased. Altogether, these findings demonstrated that the regulation of chloroplast proteome is intimately linked to light utilization, which provide basis for genetic manipulation of crop species for better adaptation and improvement of cultivation strategies.
HostingRepository	iProX
AnnounceDate	2022-09-14
AnnouncementXML	Submission_2022-09-14_19:44:16.073.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jie Zhang
SpeciesList	scientific name: Triticum aestivum; NCBI TaxID: 4565;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-14 19:43:09	ID requested
⏵ 1	2022-09-14 19:44:16	announced

Dataset with its publication pending

submitter keyword: Chloroplast proteomic analysis, Canopy light distribution, iTRAQ, photosynthetically active radiation (PAR) use efficiency (PUE), Molecular mechanism)

Xinming Ma
contact affiliation	Henan Agricultural University
contact email	maxinming@henau.edu.cn
lab head
Jie Zhang
contact affiliation	Henan Agricultural University
contact email	zhangjie135239@163.com
dataset submitter

iProX dataset URI