PXD036751

PXD036751 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis and ligand-binding studies of the odorant-binding protein OBP1 in the elephant's trunk
Description	Chemical communication in elephants has been studied in detail both at the chemical and at the behavioural levels. Several pheromones have been identified, and their specific effects on the sexual behaviour of both the African and the Asian elephants have been accurately documented. This work is focused on the characterization of OBP1 through ligand-binding studies and analysis of post-translational modifications (PTMs). First, we have performed a proteomic analysis of a crude extract of the trunk wash obtained from the African elephant, and have found OBP1 as the main component, together with minor amounts of a OBP1 isoform and the von Ebner's gland (VEG) protein. Proteomic analysis of OBP1 revealed the occurrence of a variable degree of O-glycosylation, phosphorylation and acetylation in this protein. To specifically assay OBP1 spectra of binding, we have then expressed the African elephant protein in Pichia pastoris, which showed PTMs very similar to the natural counterpart. At functional level, we have found that recombinant OBP1 from the African elephant is able to bind the sex pheromone (Z)-7-dodecenyl acetate with strong affinity and some structurally related esters with lower strength.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:43:14.721.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	CHIARA D'AMBROSIO
SpeciesList	scientific name: Loxodonta africana (African elephant); NCBI TaxID: 9785;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; complex glycosylation; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-14 14:34:32	ID requested
1	2023-03-11 10:59:17	announced
⏵ 2	2023-11-14 08:43:23	announced	2023-11-14: Updated project metadata.

Zaremska V, Renzone G, Arena S, Ciaravolo V, Buberl A, Balfanz F, Scaloni A, Knoll W, Pelosi P, An odorant-binding protein in the elephant's trunk is finely tuned to sex pheromone (Z)-7-dodecenyl acetate. Sci Rep, 12(1):19982(2022) [pubmed]

submitter keyword: odorant-binding protein

elephants

pheromones

post-translational modifications

Andrea Scaloni
contact affiliation	Proteomics, Metabolomics & Mass Spectrometry Laboratory, Institute for the Animal Production System in the Mediterranean Environment (ISPAAM), National Research Council, Piazzale Enrico Fermi 1, 80055 Portici (NA), Italy
contact email	andrea.scaloni@cnr.it
lab head
CHIARA D'AMBROSIO
contact affiliation	ISPAAM CNR
contact email	chiara.dambrosio@cnr.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD036751
     1. Label: PRIDE project
     2. Name: Proteomic analysis and ligand-binding studies of the odorant-binding protein OBP1 in the elephant's trunk