PXD036745

PXD036745 is an original dataset announced via ProteomeXchange.

Title	A simplified sample preparation for hair and skin proteins towards the application of archaeological fur and leather
Description	Traditional protocols for proteomics analysis usually start by extracting or solubilizing the proteins from their substrates. This step can be challenging for archaeological proteins, when they are heavily contaminated or decayed. The remains of animal fur/leather objects from the singer’s burial of Trossingen, an early medieval burial (580 A.D.) from Southwest Germany were submitted to proteomics analysis for species identification. One leather sample (TS3) yielded enough proteins to be identified as cow using a urea-based extraction (method “U”), confirming the microscopic identification. But two other samples (TS1 and TS2), compacted in a greyish brittle matrix with embedded hair visible only under microscope, could not be characterized with that method. A series of tests was performed using reduction/alkylation with tris(2-carboxyethyl)phosphine/chloroacetamide at 95°C directly on the matrix (method “95C”), with or without the use of paramagnetic beads as cleaning procedure (from the single-pot solidphase-enhanced sample preparation or SP3). Hair keratins were best recovered in the fur samples when digestion was performed directly on the insoluble fraction after reduction/alkylation. For both samples TS1 and TS2, an ovicaprine species was identified, with TS1 firmly identified as2 sheep due to the exceptional preservation of keratins and keratin-associated proteins. The simplified protocol also showed improvements on the identification of collagen in the leather sample TS3.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:57:43.916.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD036745
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Caroline Solazzo
SpeciesList	scientific name: Ovis aries; NCBI TaxID: 9940; scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-14 14:26:48	ID requested
1	2023-01-17 09:05:25	announced
⏵ 2	2023-11-14 08:57:44	announced	2023-11-14: Updated project metadata.

10.6019/PXD036745;

submitter keyword: Collagen,Archaeological burial, Keratin, Proteomics

Caroline Solazzo
contact affiliation	Smithsonian's Museum Conservation Institute
contact email	solazzoc@si.edu
lab head
Caroline Solazzo
contact affiliation	Smithsonian Institution
contact email	solazzoc@si.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD036745
     1. Label: PRIDE project
     2. Name: A simplified sample preparation for hair and skin proteins towards the application of archaeological fur and leather