PXD036743

PXD036743 is an original dataset announced via ProteomeXchange.

Title	Identification of SENP6 targets in HeLa cells by SENP6 SiRNA and MW shift analysis
Description	The SUMO protease SENP6 disassembles SUMO chains and thus SENP6 depletion should lead to the extension of SUMO polymers on substrates. We have used a proteomic method to identify putative SENP6 substrates based on increased apparent molecular weight after SENP6 depletion. This method uses SDS-PAGE fractionation of 6His-SUMO2 purifications from cells and slicing of gels into multiple pieces before tryptic digestion and MS analysis. Gel slices are assigned an average Apparent MW based on molecular weight standards (run in a separate lane), and this information combined with protein intensities to give every protein an Apparent MW both in the presence of SENP6 and its ablation via SiRNA. The difference between the two values allows us to understand which proteins become more SUMOylated upon SENP6 knock-down.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:32:01.502.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mike Tatham
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	ubiquitinylated lysine; sumoylated lysine; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-14 14:25:49	ID requested
1	2024-03-01 11:25:09	announced
⏵ 2	2024-10-22 06:32:01	announced	2024-10-22: Updated project metadata.

10.1016/J.CELREP.2023.112960;

submitter keyword: SUMO SENP6 Molecular Weight

Ronald T. Hay
contact affiliation	Professor of Molecular Biology University of Dundee
contact email	r.t.hay@dundee.ac.uk
lab head
Mike Tatham
contact affiliation	University of Dundee
contact email	m.tatham@dundee.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/03/PXD036743

PRIDE project URI

• PRIDE
  1. PXD036743
     1. Label: PRIDE project
     2. Name: Identification of SENP6 targets in HeLa cells by SENP6 SiRNA and MW shift analysis