PXD036736

PXD036736 is an original dataset announced via ProteomeXchange.

Title	Characterization of Chemotherapy-treated PyMT Liver Matrisome
Description	Metastasis accounts for 90% of cancer-related deaths, yet the mechanisms by which cancer cells colonize secondary organs remain poorly understood. For breast cancer patients, metastasis to the liver is associated with poor prognosis and a median survival of 6 months. Standard of care is chemotherapy, but recurrence occurs in 30% of patients. Systemic chemotherapy has been shown to induce hepatotoxicity and fibrosis, but how chemotherapy impacts the composition of the liver extracellular matrix (ECM) remains unknown. Individual ECM proteins drive tumor cell proliferation and invasion, features that are essential for metastatic outgrowth in the liver. Here, we characterize the liver ECM of tumor-bearing mice treated with and without chemotherapy using the MMTV-PyMT transgenic model of breast cancer. We identify distinct drug-specific changes induced by commonly used cytotoxic chemotherapies. We identify Collagen V as an ECM protein that is more abundant in livers isolated from paclitaxel-treated mice and identify mechanisms of Collagen V driven invasion via ECM organization and signaling through α1β1 integrins.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:58:52.961.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Justinne Guarin
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-14 14:16:46	ID requested
1	2022-10-05 07:51:45	announced
2	2023-11-14 08:35:42	announced	2023-11-14: Updated project metadata.
⏵ 3	2023-11-14 08:58:53	announced	2023-11-14: Updated project metadata.

Guarin JR, Fatherree JP, Oudin MJ, Chemotherapy treatment induces pro-invasive changes in liver ECM composition. Matrix Biol, 112():20-38(2022) [pubmed]

submitter keyword: breast cancer, mouse,Liver metastasis

Madeleine J Oudin
contact affiliation	Department of Biomedical Engineering, Tufts University, United States
contact email	madeleine.oudin@tufts.edu
lab head
Justinne Guarin
contact affiliation	Tufts University
contact email	jguari02@tufts.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD036736

PRIDE project URI

• PRIDE
  1. PXD036736
     1. Label: PRIDE project
     2. Name: Characterization of Chemotherapy-treated PyMT Liver Matrisome