PXD036728

PXD036728 is an original dataset announced via ProteomeXchange.

Title	Specific post-translational modifications of VDAC3 in ALS-SOD1 model cells identified by High-Resolution Mass Spectrometry
Description	Damage induced by oxidative stress is a key driver of the selective motor neuron death in amyotrophic lateral sclerosis (ALS). Mitochondria are among the main producers of ROS, but they also suffer particularly from their harmful effects. Voltage-dependent anion-selective channels (VDACs) are the most represented proteins of the outer mitochondrial membrane where they form pores controlling the permeation of metabolites responsible for mitochondrial functions. For these reasons, VDACs contribute to mitochondrial quality control and the entire energy metabolism of the cell. In this work we assessed whether oxidative stress in ALS induces post-translational modifications (PTMs) in VDAC3, a member of the VDAC family of outer mitochondrial membrane channel proteins, known for its role in redox signaling. At this end, protein samples enriched in VDACs were prepared from mitochondria of an ALS model cell line, NSC34 expressing human SOD1G93A, and analyzed by nUHPLC/High-Resolution nESI-MS/MS. Specific over-oxidation, deamidation, succination events were found in VDAC3 from ALS-related NSC34-SOD1G93A but not in non-ALS cell lines. We also report evidence that some PTMs may affect VDAC3 functionality. In particular, deamidation of Asn215 alone results in channel instability. Overall, our results suggest modifications of VDAC3 that can impact its protective role against ROS, which is particularly important in the ALS context.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:43:33.088.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rosaria Saletti
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue; deaminated residue; deamidated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-14 03:21:08	ID requested
1	2023-03-11 10:46:30	announced
⏵ 2	2023-11-14 08:43:41	announced	2023-11-14: Updated project metadata.

Pittal, à MGG, Reina S, Nibali SC, Cucina A, Cubisino SAM, Cunsolo V, Amodeo GF, Foti S, De Pinto V, Saletti R, Messina A, Specific Post-Translational Modifications of VDAC3 in ALS-SOD1 Model Cells Identified by High-Resolution Mass Spectrometry. Int J Mol Sci, 23(24):(2022) [pubmed]

submitter keyword: Deamidation

Amyotrophic Lateral Sclerosis

Voltage Dependent Anion Channel

post-translational modifications

mitochondria

ROS

Mass Spectrometry analysis

Orbitrap fusion tribrid

neurodegeneration

SOD1.

Rosaria Saletti
contact affiliation	Organic Mass Spectrometry Laboratory, Department of Chemical Sciences, University of Catania, Via S. Sofia 64, 95123 Catania, Italy
contact email	rsaletti@unict.it
lab head
Rosaria Saletti
contact affiliation	University of Catania
contact email	rsaletti@unict.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD036728
     1. Label: PRIDE project
     2. Name: Specific post-translational modifications of VDAC3 in ALS-SOD1 model cells identified by High-Resolution Mass Spectrometry