PXD036720

PXD036720 is an original dataset announced via ProteomeXchange.

Title	Antigen presentation deficiency, mesenchymal differentiation, and resistance to immunotherapy in the murine syngeneic CT2A tumor model
Description	A common cornerstone of preclinical cancer research is the use of syngeneic orthotopic murine tumors as immunocompetent models of human cancers. For glioblastoma research efforts, the GL261 and CT2A lines are frequently used. We systematically characterized these two lines to decipher the cell-intrinsic mechanisms that drive immuno-resistance in CT2A and to define the aspects of human cancer biology that the lines best model. We show that, despite sharing a few canonical genetic or histologic features of human glioblastoma, the transcriptional profiles of GL261 and CT2A tumours most closely resembled those of glioblastomas. CT2A additionally resembled other cancer types transcriptionally, including melanoma. CT2A displayed mesenchymal differentiation, upregulated angiogenesis, and multiple defects in antigen presentation machinery and interferon response pathways. Loss of MHC class I expression was restored in CT2A by interferon-γ treatment, explaining in part the modest efficacy of some immunotherapy combinations for CT2A. Our findings indicate that CT2A may serve as a robust preclinical solid tumor model of adaptive immune resistance.
HostingRepository	PRIDE
AnnounceDate	2023-12-19
AnnouncementXML	Submission_2023-12-19_14:03:45.279.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD036720
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ryuhjin Ahn
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-14 03:07:49	ID requested
⏵ 1	2023-12-19 14:03:45	announced

10.6019/PXD036720;

submitter keyword: CT2A,glioblastoma, immunotherapy, GL261

Forest White
contact affiliation	Massachusetts Institute of Technology
contact email	fwhite@mit.edu
lab head
Ryuhjin Ahn
contact affiliation	Massachusetts Institute of Technology
contact email	ryuhjin.ahn@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD036720
     1. Label: PRIDE project
     2. Name: Antigen presentation deficiency, mesenchymal differentiation, and resistance to immunotherapy in the murine syngeneic CT2A tumor model