PXD036714

PXD036714 is an original dataset announced via ProteomeXchange.

Title	Identification of biotinylated proteins through pull-down: ID and label-free quantification (Identification of TTP-interactome in RAW264.7 cells by TurboID proximity labelling).
Description	Identification of biotinylated proteins through pull-down. RAW264.7 cells stably expressing a Dox-inducible TurboID-TTP fusion protein were treated with LPS or Epoxomicin for 4 h, followed by 15 min of biotin labeling in the cell medium. TurboID without fusion was used as a control.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:59:05.030.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	WeiQiang Chen
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	biotinylated residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-14 03:06:02	ID requested
1	2023-03-24 00:21:44	announced
⏵ 2	2023-11-14 08:59:05	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: LPS,TTP, RAW264.7, TurboID, macrophages, Epoxomicin, biotin

Gijs Versteeg
contact affiliation	Department of Microbiology, Immunobiology and Genetics, Max Perutz Labs, University of Vienna, Vienna BioCenter (VBC), Vienna, Austria.
contact email	gijs.versteeg@univie.ac.at
lab head
WeiQiang Chen
contact affiliation	Mass Spectrometry Facility, Max Perutz Laboratories Support GmbH
contact email	chenweiqiang@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD036714

PRIDE project URI

• PRIDE
  1. PXD036714
     1. Label: PRIDE project
     2. Name: Identification of biotinylated proteins through pull-down: ID and label-free quantification (Identification of TTP-interactome in RAW264.7 cells by TurboID proximity labelling).