PXD036711

PXD036711 is an original dataset announced via ProteomeXchange.

Title	Rapid profiling of protein complex re-organization in perturbed systems
Description	Protein complexes constitute the primary functional modules of cellular activity. To respond to perturbations, complexes undergo changes in their abundance, subunit composition or state of modification. Understanding the function of biological systems requires global strategies to capture this contextual state information on protein complexes and interaction networks. Methods based on co-fractionation paired with mass spectrometry have demonstrated the capability for deep biological insight but the scope of studies using this approach has been limited by the large measurement time per biological sample and challenges with data analysis. As such, there has been little uptake of this strategy beyond a few expert labs into the broader life science community despite rich biological information content. We present a rapid integrated experimental and computational workflow to assess the re-organization of protein complexes across multiple cellular states. It enables complex experimental designs requiring increased sample/condition numbers. The workflow combines short gradient chromatography and DIA/SWATH mass spectrometry with a data analysis toolset to quantify changes in complex organization. We applied the workflow to study the global protein complex rearrangements of THP-1 cells undergoing monocyte to macrophage differentiation and a subsequent stimulation of macrophage cells with lipopolysaccharide. We observed massive proteome organization in functions related to signaling, cell adhesion, and extracellular matrix during differentiation, and less pronounced changes in processes related to innate immune response induced by the macrophage stimulation. We therefore establish our integrated differential pipeline for rapid and state-specific profiling of protein complex organization with broad utility in complex experimental designs.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_06:55:27.027.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ben Collins
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-14 03:01:43	ID requested
1	2023-06-27 13:47:55	announced
⏵ 2	2023-11-14 06:55:27	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: co-frac-ms, cofractionation, swath,protein complex, dia

Ben C. Collins
contact affiliation	School of Biological Sciences, Queen's University Belfast
contact email	ben.collins@qub.ac.ukl
lab head
Ben Collins
contact affiliation	Queen's University Belfast
contact email	ben.collins@qub.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD036711
     1. Label: PRIDE project
     2. Name: Rapid profiling of protein complex re-organization in perturbed systems