PXD036708

PXD036708 is an original dataset announced via ProteomeXchange.

Title	Structure of nucleosome-bound human PBAF complex
Description	BAF and PBAF are mammalian SWI/SNF family chromatin remodeling complexes that possess multiple histone/DNA-binding subunits and create nucleosome-depleted/free regions for transcription activation. Despite previous structural studies and recent advance of SWI/SNF family complexes, it remains incompletely understood how PBAF-nucleosome complex is organized. Here we determined structure of 13-subunit human PBAF in complex with acetylated nucleosome in ADP-BeF3-bound state. Four PBAF-specific subunits work together with nine BAF/PBAF-shared subunits to generate PBAF-specific modular organization, distinct from that of BAF at various regions. PBAF-nucleosome structure reveals six histone-binding domains and four DNA-binding domains/modules, the majority of which directly bind histone/DNA. This multivalent nucleosome-binding pattern, not observed in previous studies, suggests that PBAF may integrate comprehensive chromatin information to target genomic loci for function. Our study reveals molecular organization of subunits and histone/DNA-binding domains/modules in PBAF-nucleosome complex and provides structural insights into PBAF-mediated nucleosome association complimentary to the recently reported PBAF-nucleosome structure.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:37:47.495.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Li Wang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-14 02:38:34	ID requested
1	2023-03-10 23:07:16	announced
⏵ 2	2023-11-14 08:37:48	announced	2023-11-14: Updated project metadata.

Wang L, Yu J, Yu Z, Wang Q, Li W, Ren Y, Chen Z, He S, Xu Y, Structure of nucleosome-bound human PBAF complex. Nat Commun, 13(1):7644(2022) [pubmed]

submitter keyword: Mass spectrometry analysis of acetylated nucleosome,PBAF, NCP, LC-MS

Yanhui Xu
contact affiliation	Fudan University, Shanghai, China
contact email	xuyh@fudan.edu.cn
lab head
Li Wang
contact affiliation	Fudan University
contact email	BAIYANGLeah@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD036708
     1. Label: PRIDE project
     2. Name: Structure of nucleosome-bound human PBAF complex