PXD036704

PXD036704 is an original dataset announced via ProteomeXchange.

Title	LC-MS/MS Isomeric Profiling of N-Glycans derived from Low-Abundant Serum Glycoproteins in Mild Cognitive Im-pairment Patients
Description	Mild cognitive impairment (MCI) is an early stage of memory loss that affects cognitive abilities, such as language or virtual/spatial comprehension. This cognitive decline is mostly ob-served with the aging of individuals. Recently, MCI has been considered as a prodromal phase of Alzheimer’s disease (AD), with a 10-15% conversion rate. However, the existing diagnostic methods fail to provide precise and well-timed diagnoses, and the pathophysiology of MCI is not fully understood. Alterations of serum N-glycan expression could represent essential con-tributors to the overall pathophysiology of neurodegenerative diseases and be used as a poten-tial marker to assess MCI diagnosis using non-invasive procedures. Herein, we undertook an LC-MS/MS glycomics approach to determine and characterize potential N-glycan markers in de-pleted blood serum samples from MCI patients. For the first time, we profiled the isomeric gly-come of the low abundant serum glycoproteins extracted from serum samples of control and MCI patients using an LC-MS/MS analytical strategy. Additionally, the MRM validation of the identified data showed five isomeric N-glycans with the ability to discriminate between healthy and MCI patients: the sialylated N-glycans HexNAc5,Hex6,Neu5Ac3 and HexNAc6,Hex7,Neu5Ac4 with single AUCs of 0.92 and 0.87 respectively, and combined AUC of 0.96; and the sialylat-ed-fucosylated N-glycans HexNAc4,Hex5,Fuc,Neu5Ac, HexNAc5,Hex6,Fuc,Neu5Ac2 and HexNAc6,Hex7,Fuc,Neu5Ac3 with single AUCs of 0.94, 0.67, and 0.88 respectively, and combined AUC of 0.98. According to ingenuity pathway analysis (IPA) and in line with recent publications the identified N-glycans may play an important role in neuroinflammation. Process that plays a fundamental role in the progression of neurodegenerative diseases.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:43:57.359.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD036704
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Cristian Gutierrez Reyes
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos; TSQ Vantage

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-14 02:30:17	ID requested
1	2023-03-11 11:07:22	announced
⏵ 2	2023-11-14 08:43:59	announced	2023-11-14: Updated project metadata.

10.6019/PXD036704;

Reyes CDG, Hakim MA, Atashi M, Goli M, Gautam S, Wang J, Bennett AI, Zhu J, Lubman DM, Mechref Y, -Glycans Derived from Low-Abundant Serum Glycoproteins in Mild Cognitive Impairment Patients. Biomolecules, 12(11):(2022) [pubmed]

submitter keyword: MCI, biomarker glycan, Neurological disorder, Isomeric N-glycans

Yehia Mechref
contact affiliation	Texas Tech University
contact email	yehia.mechref@ttu.edu
lab head
Cristian Gutierrez Reyes
contact affiliation	Graduate student
contact email	cristian.d.gutierrez-reyes@ttu.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD036704

PRIDE project URI

• PRIDE
  1. PXD036704
     1. Label: PRIDE project
     2. Name: LC-MS/MS Isomeric Profiling of N-Glycans derived from Low-Abundant Serum Glycoproteins in Mild Cognitive Im-pairment Patients