PXD036701

PXD036701 is an original dataset announced via ProteomeXchange.

Title	Proteomic study of a breast cancer cell line following hemin treatment
Description	Heme, an iron-containing prosthetic group found in many proteins, carries out diverse biological functions such as electron transfer, oxygen storage and enzymatic reactions. Hemin, the oxidised form of heme, is used to treat porphyria and also to activate heme-oxygenase (HO) which catalyses the rate-limiting step in heme degradation. Our group has previously demonstrated that hemin displays antitumor activity in breast cancer (BC). The aim of this work has been to study the effect of hemin on protein expression modifications in a BC cell line to gain insight into the molecular mechanisms of hemin antitumor activity. For this purpose, we carried out proteome analysis by Mass Spectrometry (MS) which showed that 1309 proteins were significantly increased in hemin-treated cells, including HO-1 and the proteases that regulate HO-1 function, and 921 proteins were significantly decreased. Furthermore, the MS-data analysis showed that hemin regulates the expression of heme- and iron- related proteins, adhesion and cytoskeletal proteins, cancer signal transduction proteins and enzymes involved in lipid metabolism. By biochemical and cellular studies, we further corroborated the most relevant in-silico results. Altogether, these results show the multiple physiological effects that hemin treatment displays in BC and demonstrate its potential as anticancer agent.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:03:10.253.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	GEORGINA COLO
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Thermo Scientific instrument model

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-14 02:23:55	ID requested
1	2023-07-20 11:21:40	announced
⏵ 2	2023-11-14 09:03:18	announced	2023-11-14: Updated project metadata.

Col, ó GP, Schweitzer K, Oresti GM, Alonso EG, Ch, á, vez LF, Mascar, ó M, Giorgi G, Curino AC, Facchinetti MM, Proteomic analysis of the effect of hemin in breast cancer. Sci Rep, 13(1):10091(2023) [pubmed]

submitter keyword: Hemin, Proteomic, Mass spectrometry,Breast cancer, Heme oxygenase-1

Alejandro Curino
contact affiliation	Cancer Biology laboratory, INIBIBB_UNS_CONICET
contact email	acurino@criba.edu.ar
lab head
GEORGINA COLO
contact affiliation	CONICET
contact email	gcolo@inibibb-conicet.gob.ar
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD036701

PRIDE project URI

• PRIDE
  1. PXD036701
     1. Label: PRIDE project
     2. Name: Proteomic study of a breast cancer cell line following hemin treatment