PXD036693

PXD036693 is an original dataset announced via ProteomeXchange.

Title	LC-MS/MS identification of SIRT2 interaction proteins
Description	HEK293T cells transfected with plasmids expressing Flag-Luc or Flag-SIRT2 were lysed in CHAPS lysis buffer (120 mM NaCl, 0.3% CHAPS (Sigma, C900480), 1 mM EDTA, 40 mM HEPES (pH 7.5), and complete protease inhibitor cocktail (Roche)) at 4 °C for 2 h with rotation, and then centrifuged at 12 000 g at 4°C for 30 min. For Co-IP with exogenous proteins, the resulting supernatants were mixed with anti-FLAG Affinity Gel (Sigma, A2220) and rotated overnight at 4 °C. The SIRT2 interacting protein complexes by anti-Flag Affinity Gel were competitively eluted using FLAG peptides and processed for Western blotting analysis.
HostingRepository	iProX
AnnounceDate	2022-09-13
AnnouncementXML	Submission_2022-10-13_19:31:44.853.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kuan Yang
SpeciesList	scientific name: Macaca fascicularis; NCBI TaxID: 9541;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-13 19:24:54	ID requested
⏵ 1	2022-10-13 19:31:45	announced

Dataset with its publication pending

submitter keyword: LC-MS/MS, co-IP

Weiqi Zhang
contact affiliation	Beijing Institute of Genomics, Chinese Academy of Sciences
contact email	zhangwq@big.ac.cn
lab head
Kuan Yang
contact affiliation	Beijing Institute of Genomics, Chinese Academy of Sciences
contact email	yangkuan@big.ac.cn
dataset submitter

iProX dataset URI