PXD036680

PXD036680 is an original dataset announced via ProteomeXchange.

Title	Proteomes of ZIP8-wildtype and ZIP8-KO HeLa cell lines
Description	The human ZRT/IRT-like protein 8 (ZIP8), encoded by the solute carrier family 39 member 8 gene SLC39A8, is a metal cation symporter located mainly on the cell membrane. ZIP8 is well-known for its role in transporting divalent metal ions into the cells – these ions include several essential micronutrients (e.g., iron [Fe], manganese [Mn], and zinc [Zn]) and non-essential toxic heavy metal cadmium (Cd). In the current project, ZIP8 gene in the human cervical cancer HeLa cell line was knockout (KO) using the CRISPR/Cas9 genome editing technology. Then, the proteomes of ZIP8-KO HeLa cell line and HeLa parental cell line (with wildtype ZIP8 gene sequence) were quantified using iTRAQ and LC-MS/MS.
HostingRepository	PRIDE
AnnounceDate	2022-10-20
AnnouncementXML	Submission_2022-10-20_02:39:26.513.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD036680
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Heng WeeTan
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-12 07:02:16	ID requested
⏵ 1	2022-10-20 02:39:26	announced

10.6019/PXD036680;

submitter keyword: single-gene knockout-coupled omics, SLC39A8, HeLa cell line,ZIP8, iTRAQ

Andy T. Y.Lau
contact affiliation	Laboratory of Cancer Biology and Epigenetics, Department of Cell Biology and Genetics, Shantou University Medical College, Shantou, 515041, Guangdong, People’s Republic of China
contact email	andytylau@stu.edu.cn
lab head
Heng WeeTan
contact affiliation	Shantou University Medical College
contact email	hwtan@stu.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD036680

PRIDE project URI

• PRIDE
  1. PXD036680
     1. Label: PRIDE project
     2. Name: Proteomes of ZIP8-wildtype and ZIP8-KO HeLa cell lines