PXD036664

PXD036664 is an original dataset announced via ProteomeXchange.

Title	Three types of Leishmania mexicana amastigotes: proteome comparison by quantitative proteomic analysis
Description	Leishmania is the unicellular parasite transmitted by phlebotomine sand fly bite. It exists in two different forms; extracellular promastigotes, occurring in the gut of sand flies, and intracellular, round-shaped amastigotes residing mainly in vertebrate macrophages. As amastigotes originating from infected animals are often present in insufficient quality and quantity, two alternative types of amastigotes were introduced for laboratory experiments: axenic amastigotes and amastigotes from macrophages infected in vitro. Nevertheless, there is very little information about the degree of similarity/difference among these three types of amastigotes on proteomic level, whose comparison is crucial for assessing the suitability of using alternative types of amastigotes in experiments. In this study, L. mexicana amastigotes obtained from lesion of infected BALB/c mice were proteomically compared with alternatively cultivated amastigotes (axenic and macrophage-derived ones). Amastigotes of all three types were isolated, individually treated and analysed by LC-MS/MS proteomic analysis with quantification using TMT10-plex isobaric labeling. Significant differences were observed in the abundance of metabolic enzymes, virulence factors and proteins involved in translation and condensation of DNA. The most pronounced differences were observed between axenic amastigotes and lesion-derived amastigotes, macrophage-derived amastigotes were mostly intermediate between axenic and lesion-derived ones.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:42:47.478.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD036664
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Karel Harant
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-12 06:33:13	ID requested
1	2023-03-11 04:42:15	announced
⏵ 2	2023-11-14 08:42:55	announced	2023-11-14: Updated project metadata.

Pacakova L, Harant K, Volf P, Lestinova T, amastigotes: Proteome comparison by quantitative proteomic analysis. Front Cell Infect Microbiol, 12():1022448(2022) [pubmed]

10.6019/PXD036664;

submitter keyword: amastigotes,L. mexicana

Karel Harant
contact affiliation	Laboratory of Mass Spectrometry, BIOCEV, Faculty of Science Charles University in Prague
contact email	harant@natur.cuni.cz
lab head
Karel Harant
contact affiliation	Charles University
contact email	harant@natur.cuni.cz
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD036664
     1. Label: PRIDE project
     2. Name: Three types of Leishmania mexicana amastigotes: proteome comparison by quantitative proteomic analysis