PXD036652

PXD036652 is an original dataset announced via ProteomeXchange.

Title	Earliest changes associated with ALS detected in serum EVs.
Description	Amyotrophic Lateral Sclerosis (ALS) is a neurodegenerative disease characterized by the deposition of mutated and misfolded proteins leading to the degradation of motor neurons and the motor cortex. Several ALS-associated proteins have been linked to small extracellular vesicles (EVs). However, the role of these EVs and their cargo in the early stages of ALS has not been investigated. This study aims to identify the earliest protein changes facilitated by EVs in ALS by examining the serum of newly diagnosed ALS patients. EVs were isolated from the serum of ALS (n = 15) and healthy control (HC, n = 15) patients, before undergoing mass spectrometry analysis resulting in the identification of a panel of proteins associated with the early changes of ALS. This panel consists of 9 statistically significantly up-regulated proteins and includes haptoglobin and hemoglobin subunits, complement, and afamin, which are involved in pathways including: heme homeostasis and autophagy. The identification of haptoglobin in the ALS serum EVs suggests it has potential as an early diagnostic biomarker whilst, activation of autophagy pathways suggests early recruitment of clearance pathways in ALS. Therefore, this study uncovers the processes and proteins being facilitated through small EVs in the initial stages of ALS.
HostingRepository	PRIDE
AnnounceDate	2024-03-13
AnnouncementXML	Submission_2024-03-13_16:13:19.550.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rohan Lowe
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-12 06:19:36	ID requested
⏵ 1	2024-03-13 16:13:19	announced

10.1101/2023.07.26.23292854;

submitter keyword: Amyotrophic Lateral Sclerosis, Serum, Extracellular Vesicles, Liquid Biopsy, Biomarkers

Lesley Cheng
contact affiliation	The Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Bundoora, Victoria, Australia
contact email	l.cheng@latrobe.edu.au
lab head
Rohan Lowe
contact affiliation	La Trobe University
contact email	r.lowe@latrobe.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/03/PXD036652

PRIDE project URI

• PRIDE
  1. PXD036652
     1. Label: PRIDE project
     2. Name: Earliest changes associated with ALS detected in serum EVs.