PXD036650

PXD036650 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Identification of Cannabidiol interactome in K562 cells
Description	Cannabidiol (CBD) showed antiproliferative activity and induction of apoptosis in chronic myelogenous leukaemia cancer cells (K562 cells). A consolidated DARTS (Drug Affinity Response Target Stability) and t-LIP-MRM (targeted- Limited Proteolysis-Multiple Reaction Monitoring Mass spectrometry) platform was applied to unveil the interactome of CBD and to shed light on its mechanism of action. Both approaches point to the identification of DNA topoisomerase 2-alpha (TOP2A) as a major CBD target in K562 cells.
HostingRepository	PRIDE
AnnounceDate	2024-01-26
AnnouncementXML	Submission_2024-01-26_07:17:02.401.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sara Ceccacci
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-12 06:16:55	ID requested
⏵ 1	2024-01-26 07:17:03	announced

Publication List

10.1016/j.heliyon.2024.e24196;
Ceccacci S, Corsi L, Spinelli L, Caroli C, Marani M, Anceschi L, Mozzicafreddo M, Pellati F, Monti MC, A label free chemoproteomic-based platform to disclose cannabidiol molecular mechanism of action on chronic myelogenous leukemia cancer cells. Heliyon, 10(1):e24196(2024) [pubmed]

10.1016/j.heliyon.2024.e24196;

Ceccacci S, Corsi L, Spinelli L, Caroli C, Marani M, Anceschi L, Mozzicafreddo M, Pellati F, Monti MC, A label free chemoproteomic-based platform to disclose cannabidiol molecular mechanism of action on chronic myelogenous leukemia cancer cells. Heliyon, 10(1):e24196(2024) [pubmed]

Keyword List

submitter keyword: CBD
K562 cells
TOP2A
DARTS
t-LiP-MRM
chemoproteomics
mass spectrometry

submitter keyword: CBD

K562 cells

TOP2A

DARTS

t-LiP-MRM

chemoproteomics

mass spectrometry

Contact List

Maria Chiara Monti
contact affiliation	Department of Pharmacy, University of Salerno, 84084 Fisciano, Italy
contact email	mcmonti@unisa.it
lab head
Sara Ceccacci
contact affiliation	Department of Pharmacy, University of Salerno, 84084 Fisciano
contact email	sceccacci@unisa.it
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD036650
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD036650

PRIDE project URI

Repository Record List

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