PXD036639

PXD036639 is an original dataset announced via ProteomeXchange.

Title	Advanced physiological maturation of iPSC-derived human cardiomyocytes using an algorithm-directed optimization of defined media components
Description	Induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) hold tremendous promise for in vitro modeling 2 to assess native myocardial function and disease mechanisms as well as testing drug safety and efficacy. However, 3 current iPSC-CMs are functionally immature, resembling in vivo CMs of fetal or neonatal developmental states. The 4 use of targeted culture media and organoid formats have been identified as potential high-yield contributors to 5 improve CM maturation. This study presents a novel iPSC-CM maturation medium formulation, designed using a 6 differential evolutionary approach targeting metabolic functionality for iterative optimization. Relative to gold-7 standard reference formulations, our medium significantly matured morphology, Ca2+ handling, electrophysiology, 8 and metabolism, which was further validated by multi-omic screening, for cells in either pure or co-cultured 9 microtissue formats. Together, these findings not only provide a reliable workflow for highly functional iPSC-CMs 10 for downstream use, but also demonstrate the power of high-dimensional optimization processes in evoking 11 advanced biological function in vitro.
HostingRepository	PRIDE
AnnounceDate	2026-02-16
AnnouncementXML	Submission_2026-02-16_14:20:41.418.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Uros Kuzmanov
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-12 05:24:39	ID requested
⏵ 1	2026-02-16 14:20:41	announced

10.1101/2022.10.10.507929;

submitter keyword: electrophysiology, metabolism, contractility, proteomics, screening,myocardium, tissue engineering, RNA sequencing

Craig Simmons
contact affiliation	Translational Biology and Engineering Program, Ted Rogers Centre for Heart Research, Toronto, Ontario M5G 1M1, Canada
contact email	simmons@mie.utoronto.ca
lab head
Uros Kuzmanov
contact affiliation	University of Toronto
contact email	uros.kuzmanov@utoronto.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD036639

PRIDE project URI

• PRIDE
  1. PXD036639
     1. Label: PRIDE project
     2. Name: Advanced physiological maturation of iPSC-derived human cardiomyocytes using an algorithm-directed optimization of defined media components