PXD036630 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A photo-oxidation driven proximity labeling strategy enables profiling of mitochondrial proteome dynamics in living cells |
Description | We herein report a photo-oxidation driven proximity labeling strategy to profile mitochondrial proteome by light dependence in living cells with high spatiotemporal resolution. Taking advantage of organelle-localizable organic photoactivated probe generating reactive species and nucleophilic substrate for proximal proteins oxidation and trapping, mitochondrial proteins were selectively labeled by spatially limited reactions in native environment. Integration of photo-oxidation driven proximity labeling and quantitative proteomics facilitated the plotting of mitochondrial proteome in which up to 310 mitochondrial proteins were identified with the specificity of 64% in HeLa cells. Furthermore, mitochondrial proteome dynamics was deciphered in drug resistant Huh7 and LPS stimulated HMC3 cells which were hard-to-transfect. A number of differential proteins were quantified which were intimately linked to critical processes and provided insights into the related molecular mechanisms of drug resistance and neuroinflammation in the perspective of mitochondria. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:40:50.857.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | He Wang |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion Lumos; Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-09-12 05:21:26 | ID requested | |
1 | 2023-03-11 02:28:25 | announced | |
⏵ 2 | 2023-11-14 08:40:54 | announced | 2023-11-14: Updated project metadata. |
Publication List
Wang H, Wang Z, Gao H, Liu J, Qiao Z, Zhao B, Liang Z, Jiang B, Zhang L, Zhang Y, A photo-oxidation driven proximity labeling strategy enables profiling of mitochondrial proteome dynamics in living cells. Chem Sci, 13(40):11943-11950(2022) [pubmed] |
Keyword List
submitter keyword: Photo-oxidation, proximity labeling, mitochondrial proteome |
Contact List
He Wang |
contact affiliation | CAS Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic R & A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China. |
contact email | crane_wanghe@dicp.ac.cn |
lab head | |
He Wang |
contact affiliation | Dalian Institute of Chemical Physics, Chinese Academy of Sciences |
contact email | crane_wanghe@dicp.ac.cn |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD036630
- Label: PRIDE project
- Name: A photo-oxidation driven proximity labeling strategy enables profiling of mitochondrial proteome dynamics in living cells