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PXD036630

PXD036630 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA photo-oxidation driven proximity labeling strategy enables profiling of mitochondrial proteome dynamics in living cells
DescriptionWe herein report a photo-oxidation driven proximity labeling strategy to profile mitochondrial proteome by light dependence in living cells with high spatiotemporal resolution. Taking advantage of organelle-localizable organic photoactivated probe generating reactive species and nucleophilic substrate for proximal proteins oxidation and trapping, mitochondrial proteins were selectively labeled by spatially limited reactions in native environment. Integration of photo-oxidation driven proximity labeling and quantitative proteomics facilitated the plotting of mitochondrial proteome in which up to 310 mitochondrial proteins were identified with the specificity of 64% in HeLa cells. Furthermore, mitochondrial proteome dynamics was deciphered in drug resistant Huh7 and LPS stimulated HMC3 cells which were hard-to-transfect. A number of differential proteins were quantified which were intimately linked to critical processes and provided insights into the related molecular mechanisms of drug resistance and neuroinflammation in the perspective of mitochondria.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:40:50.857.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterHe Wang
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion Lumos; Orbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-09-12 05:21:26ID requested
12023-03-11 02:28:25announced
22023-11-14 08:40:54announced2023-11-14: Updated project metadata.
Publication List
Wang H, Wang Z, Gao H, Liu J, Qiao Z, Zhao B, Liang Z, Jiang B, Zhang L, Zhang Y, A photo-oxidation driven proximity labeling strategy enables profiling of mitochondrial proteome dynamics in living cells. Chem Sci, 13(40):11943-11950(2022) [pubmed]
Keyword List
submitter keyword: Photo-oxidation, proximity labeling, mitochondrial proteome
Contact List
He Wang
contact affiliationCAS Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic R & A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
contact emailcrane_wanghe@dicp.ac.cn
lab head
He Wang
contact affiliationDalian Institute of Chemical Physics, Chinese Academy of Sciences
contact emailcrane_wanghe@dicp.ac.cn
dataset submitter
Full Dataset Link List
Dataset FTP location
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