PXD036626

PXD036626 is an original dataset announced via ProteomeXchange.

Title	TAP and nano LC-MS/MS of Arabidopsis thaliana ABCE2 protein
Description	ATP-Binding Cassette E (ABCE) proteins dissociate cytoplasmic ribosomes after translation terminates, and contribute to ribosome recycling, thus linking translation termination to initiation. This function has been demonstrated to be essential in animals, fungi, and archaea, but remains unexplored in plants. In most species, ABCE is encoded by a single-copy gene; by contrast, Arabidopsis thaliana has two ABCE paralogs, of which ABCE2 seems to conserve the ancestral function. We isolated apiculata7-1 (api7-1), the first viable, hypomorphic allele of ABCE2, which has a pleiotropic morphological phenotype reminiscent of mutations affecting ribosome biogenesis factors and ribosomal proteins. We also studied api7-2, a null, recessive lethal allele of ABCE2. Co-immunoprecipitation experiments showed that ABCE2 physically interacts with components of the translation machinery. An RNA-seq study of the api7-1 mutant showed increased responses to iron and sulfur starvation. We also found increased transcript levels of genes related to auxin signaling and metabolism. Our results support for the first time a conserved role for ABCE proteins in translation in plants, as previously shown for the animal, fungal, and archaeal lineages. In Arabidopsis, the ABCE2 protein seems important for general growth and vascular development, likely due to an indirect effect through auxin metabolism.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:38:35.095.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Carla Navarro-Quiles
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-12 03:55:53	ID requested
1	2022-11-15 07:23:52	announced
⏵ 2	2023-11-14 07:38:36	announced	2023-11-14: Updated project metadata.

Navarro-Quiles C, Mateo-Bonmat, í E, Candela H, Robles P, Mart, í, nez-Laborda A, Fern, á, ndez Y, Š, imura J, Ljung K, Rubio V, Ponce MR, Micol JL, The Arabidopsis ATP-Binding Cassette E protein ABCE2 is a conserved component of the translation machinery. Front Plant Sci, 13():1009895(2022) [pubmed]

submitter keyword: TAP

LC-MS/MS

Arabidopsis

ABCE2

translation

José Luis Micol
contact affiliation	Instituto de Bioingeniería, Universidad Miguel Hernández, 03202 Elche, SPAIN
contact email	jlmicol@umh.es
lab head
Carla Navarro-Quiles
contact affiliation	Universidad Miguel Hernández
contact email	carla.navarroq@umh.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/11/PXD036626

PRIDE project URI

• PRIDE
  1. PXD036626
     1. Label: PRIDE project
     2. Name: TAP and nano LC-MS/MS of Arabidopsis thaliana ABCE2 protein